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Bioengineering of microbial transglutaminase for biomedical applications

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Abstract

Microbial transglutaminase (mTGase) is commonly known in the food industry as meat glue due to its incredible ability to “glue” meat proteins together. Aside from being widely exploited in the meat processing industries, mTGase is also widely applied in other food and textile industries by catalysing the formation of isopeptide bonds between peptides or protein substrates. The advancement of technology has opened up new avenues for mTGase in the field of biomedical engineering. Efforts have been made to study the structural properties of mTGase in order to gain an in-depth understanding of the structure-function relationship. This review highlights the developments in mTGase engineering together with its role in biomedical applications including biomaterial fabrication for tissue engineering and biotherapeutics.

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Funding

This study was funded by Malaysian Ministry of Education under the Higher Institutions Centre of Excellence (HICoE) Grant Scheme (311/CIPPM/4401005).

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  1. Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800, Penang, Malaysia

    Soo Khim Chan & Theam Soon Lim

  2. Analytical Biochemistry Research Centre, Universiti Sains Malaysia, 11800, Penang, Malaysia

    Theam Soon Lim

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  1. Soo Khim Chan
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Correspondence to Soo Khim Chan.

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Chan, S.K., Lim, T.S. Bioengineering of microbial transglutaminase for biomedical applications. Appl Microbiol Biotechnol 103, 2973–2984 (2019). https://doi.org/10.1007/s00253-019-09669-3

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