The present study addresses the synthesis and properties of polyhydroxyalkanoates (PHA) of different composition synthesized by Cupriavidus eutrophus B-10646 using glycerol as a carbon substrate. Poly(3-hydroxybutyrate) [P(3HB)] was effectively synthesized in fed-batch culture in a 30-L fermenter on glycerol of various purification degrees, with 99.5, 99.7, and 82.1% content of the main component. Purified glycerol (99.7%) was used for 150-L pilot scale fermentation. The total biomass and P(3HB) concentration reached 110 and 85.8 g/L, respectively, after 45 h of fed-batch fermentation. An average volumetric productivity of P(3HB) was 1.83 g/(L h). The degree of crystallinity and molecular weight of P(3HB) synthesized on glycerol were lower than and temperature characteristics were the same as those of P(3HB) synthesized on sugars.
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This study was financially supported by the project “Agropreparations of the new generation: a strategy of construction and realization” (Agreement No. 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, “On measures designed to attract leading scientists to the Russian institutions of higher learning”.
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This article does not contain any studies with human participants or animals, performed by any of the authors.
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Volova, T., Demidenko, A., Kiselev, E. et al. Polyhydroxyalkanoate synthesis based on glycerol and implementation of the process under conditions of pilot production. Appl Microbiol Biotechnol 103, 225–237 (2019). https://doi.org/10.1007/s00253-018-9460-0