Applied Microbiology and Biotechnology

, Volume 103, Issue 1, pp 225–237 | Cite as

Polyhydroxyalkanoate synthesis based on glycerol and implementation of the process under conditions of pilot production

  • Tatiana Volova
  • Aleksey Demidenko
  • Evgeniy Kiselev
  • Sergey Baranovskiy
  • Ekaterina Shishatskaya
  • Natalia ZhilaEmail author
Biotechnological products and process engineering


The present study addresses the synthesis and properties of polyhydroxyalkanoates (PHA) of different composition synthesized by Cupriavidus eutrophus B-10646 using glycerol as a carbon substrate. Poly(3-hydroxybutyrate) [P(3HB)] was effectively synthesized in fed-batch culture in a 30-L fermenter on glycerol of various purification degrees, with 99.5, 99.7, and 82.1% content of the main component. Purified glycerol (99.7%) was used for 150-L pilot scale fermentation. The total biomass and P(3HB) concentration reached 110 and 85.8 g/L, respectively, after 45 h of fed-batch fermentation. An average volumetric productivity of P(3HB) was 1.83 g/(L h). The degree of crystallinity and molecular weight of P(3HB) synthesized on glycerol were lower than and temperature characteristics were the same as those of P(3HB) synthesized on sugars.


Glycerol Polyhydroxyalkanoates Synthesis Productivity Properties 



This study was financially supported by the project “Agropreparations of the new generation: a strategy of construction and realization” (Agreement No. 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, “On measures designed to attract leading scientists to the Russian institutions of higher learning”.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals, performed by any of the authors.

Supplementary material

253_2018_9460_MOESM1_ESM.pdf (395 kb)
ESM 1 (PDF 395 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Tatiana Volova
    • 1
    • 2
  • Aleksey Demidenko
    • 1
    • 2
  • Evgeniy Kiselev
    • 1
    • 2
  • Sergey Baranovskiy
    • 1
  • Ekaterina Shishatskaya
    • 1
    • 2
  • Natalia Zhila
    • 1
    • 2
    Email author
  1. 1.Siberian Federal UniversityKrasnoyarskRussian Federation
  2. 2.Institute of Biophysics SB RASFederal Research Center “Krasnoyarsk Science Center SB RAS”KrasnoyarskRussian Federation

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