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Production of d-lactic acid by Lactobacillus delbrueckii ssp. delbrueckii from orange peel waste: techno-economical assessment of nitrogen sources

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Abstract

In this study, the effect of several organic nitrogen sources (namely peptone, meat extract—ME, yeast extract—YE, and corn steep liquor—CSL) on d-lactic acid production by Lactobacillus delbrueckii ssp. delbrueckii has been studied. While lactic acid bacteria (LAB) are well-known for their complex nutritional requirements, organic nitrogen source-related cost can be as high as 38% of total operational costs (OPEX), being its nature and concentration critical factors in the growth and productivity of the selected strain. Corn steep liquor (CSL) has been chosen for its adequacy, on the grounds of the d-lactic acid yield, productivity, and its cost per kilogram of product. Finally, orange peel waste hydrolysate supplemented with 37 g/l CSL has been employed for d-lactic acid production, reaching a final yield of 88% and a productivity of 2.35 g/l h. CSL cost has been estimated at 90.78$/ton of d-lactate.

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References

  • Abdel-Rahman MA, Tashiro Y, Sonomoto K (2011) Lactic acid production from lignocellulose-derived sugars using lactic acid bacteria: overview and limits. J Biotechnol 156(4):286–301

    Article  CAS  Google Scholar 

  • Abdel-Rahman MA, Tashiro Y, Sonomoto K (2013) Recent advances in lactic acid production by microbial fermentation processes. Biotechnol Adv 31(6):877–902

    Article  CAS  Google Scholar 

  • Assavasirijinda N, Ge D, Yu B, Xue Y, Ma Y (2016) Efficient fermentative production of polymer-grade d-lactate by an engineered alkaliphilic Bacillus sp. strain under non-sterile conditions. Microb Cell Factories 15(1):3

    Article  Google Scholar 

  • Bevilacqua A, Corbo MR, Sinigaglia M (2010) In vitro evaluation of the antimicrobial activity of eugenol, limonene, and citrus extract against bacteria and yeasts, representative of the spoiling microflora of fruit juices. J Food Prot 73(5):888–894

    Article  CAS  Google Scholar 

  • Calabia BP, Tokiwa Y (2007) Production of D-lactic acid from sugarcane molasses, sugarcane juice and sugar beet juice by Lactobacillus delbrueckii. Biotechnol Lett 29(9):1329–1332

    Article  CAS  Google Scholar 

  • Choi M, Al-Zahrani SM, Lee SY (2014) Kinetic model-based feed-forward controlled fed-batch fermentation of Lactobacillus rhamnosus for the production of lactic acid from Arabic date juice. Bioprocess Biosyst Eng 37(6):1007–1015

    Article  CAS  Google Scholar 

  • Cote RJ (2010) Media composition, microbial, laboratory scale. In: Flickinger MC (ed) Encyclopedia of industrial biotechnology: bioprocess, bioseparation, and cell technology. John Wiley & Sons, Hoboken, pp 1–20

  • de la Torre I, Ravelo M, Segarra S, Tortajada M, Santos VE, Ladero M (2017) Study on the effects of several operational variables on the enzymatic batch saccharification of orange solid waste. Bioresour Technol 245(Part A):906–915

    Article  Google Scholar 

  • de Oliveira Moraes A, Ramirez NIB, Pereira N (2016) Evaluation of the fermentation potential of pulp mill residue to produce D (−)-lactic acid by separate hydrolysis and fermentation using Lactobacillus coryniformis subsp. torquens. Appl Biochem Biotechnol 180(8):1574–1585

    Article  CAS  Google Scholar 

  • Eiteman MA, Ramalingam S (2015) Microbial production of lactic acid. Biotechnol Lett 37(5):955–972

    Article  CAS  Google Scholar 

  • Esteban J, Ladero M (2018) Food waste as a source of value-added chemicals and materials: a biorefinery perspective. Int J Food Sci Technol 53(5):1095–1108

    Article  CAS  Google Scholar 

  • Girotto F, Alibardi L, Cossu R (2015) Food waste generation and industrial uses: a review. Waste Manag 45:32–41

    Article  CAS  Google Scholar 

  • Gullon B, Yánez R, Alonso JL, Parajo JC (2008) L-Lactic acid production from apple pomace by sequential hydrolysis and fermentation. Bioresour Technol 99(2):308–319

    Article  Google Scholar 

  • Hama S, Mizuno S, Kihara M, Tanaka T, Ogino C, Noda H, Kondo A (2015) Production of D-lactic acid from hardwood pulp by mechanical milling followed by simultaneous saccharification and fermentation using metabolically engineered Lactobacillus plantarum. Bioresour Technol 187:167–172

    Article  CAS  Google Scholar 

  • Hebert EM, Raya RR, De Giori GS (2000) Nutritional requirements and nitrogen-dependent regulation of proteinase activity of Lactobacillus helveticus CRL 1062. Appl Microbiol Biotechnol 66(12):5316–5321

    CAS  Google Scholar 

  • Hofvendahl K, Hahn-Hägerdal B (2000) Factors affecting the fermentative lactic acid production from renewable resources. Enzym Microb Technol 26(2):87–107

    Article  CAS  Google Scholar 

  • Hu J, Zhang Z, Lin Y, Zhao S, Mei Y, Liang Y, Peng N (2015) High-titer lactic acid production from NaOH-pretreated corn stover by Bacillus coagulans LA204 using fed-batch simultaneous saccharification and fermentation under non-sterile condition. Bioresour Technol 182:251–257

    Article  CAS  Google Scholar 

  • Idris A, Suzana W (2006) Effect of sodium alginate concentration, bead diameter, initial pH and temperature on lactic acid production from pineapple waste using immobilized Lactobacillus delbrueckii. Process Biochem 41(5):1117–1123

    Article  CAS  Google Scholar 

  • John RP, Nampoothiri KM, Pandey A (2007) Fermentative production of lactic acid from biomass: an overview on process developments and future perspectives. Appl Microbiol Biotechnol 74(3):524–534

    Article  CAS  Google Scholar 

  • John RP, Anisha G, Nampoothiri KM, Pandey A (2009) Direct lactic acid fermentation: focus on simultaneous saccharification and lactic acid production. Biotechnol Adv 27(2):145–152

    Article  CAS  Google Scholar 

  • Klotz S, Kaufmann N, Kuenz A, Prüße U (2016) Biotechnological production of enantiomerically pure d-lactic acid. Appl Microbiol Biotechnol 100(22):9423–9437

    Article  CAS  Google Scholar 

  • Klotz S, Kuenz A, Prüße U (2017) Nutritional requirements and the impact of yeast extract on the d-lactic acid production by Sporolactobacillus inulinus. Green Chem 19(19):4633–4641

    Article  CAS  Google Scholar 

  • Kwan TH, Hu Y, Lin CSK (2016) Valorisation of food waste via fungal hydrolysis and lactic acid fermentation with Lactobacillus casei Shirota. Bioresour Technol 217:129–136

    Article  CAS  Google Scholar 

  • Kwon S, Lee PC, Lee EG, Chang YK, Chang N (2000) Production of lactic acid by Lactobacillus rhamnosus with vitamin-supplemented soybean hydrolysate. Enzym Microb Technol 26(2–4):209–215

    Article  CAS  Google Scholar 

  • Lu Z, Lu M, He F, Yu L (2009) An economical approach for D-lactic acid production utilizing unpolished rice from aging paddy as major nutrient source. Bioresour Technol 100(6):2026–2031

    Article  CAS  Google Scholar 

  • Man JCD, Rogosa M, Sharpe EM (1960) A medium for the cultivation of Lactobacilli. J Appl Bacteriol 23(1):130–135

    Article  Google Scholar 

  • Mazzoli R, Bosco F, Mizrahi I, Bayer EA, Pessione E (2014) Towards lactic acid bacteria-based biorefineries. Biotechnol Adv 32(7):1216–1236

    Article  CAS  Google Scholar 

  • Nancib A, Nancib N, Meziane-Cherif D, Boubendir A, Fick M, Boudrant J (2005) Joint effect of nitrogen sources and B vitamin supplementation of date juice on lactic acid production by Lactobacillus casei subsp. rhamnosus. Bioresour Technol 96(1):63–67

    Article  CAS  Google Scholar 

  • Negro V, Mancini G, Ruggeri B, Fino D (2016) Citrus waste as feedstock for bio-based products recovery: review on limonene case study and energy valorization. Bioresour Technol 214:806–815. https://doi.org/10.1016/j.biortech.2016.05.006

    Article  CAS  PubMed  Google Scholar 

  • Nguyen CM, Choi GJ, Choi YH, Jang KS, Kim J-C (2013) D-and L-lactic acid production from fresh sweet potato through simultaneous saccharification and fermentation. BiochemEngJ 81:40–46

    CAS  Google Scholar 

  • Okano K, Hama S, Kihara M, Noda H, Tanaka T, Kondo A (2017) Production of optically pure d-lactic acid from brown rice using metabolically engineered Lactobacillus plantarum. Appl Microbiol Biotechnol 101(5):1869–1875

    Article  CAS  Google Scholar 

  • Papadimitriou K, Alegría Á, Bron PA, De Angelis M, Gobbetti M, Kleerebezem M, Lemos JA, Linares DM, Ross P, Stanton C (2016) Stress physiology of lactic acid bacteria. Microbiol Mol Biol Rev 80(3):837–890

    Article  CAS  Google Scholar 

  • Prasad S, Srikanth K, Limaye AM, Sivaprakasam S (2014) Homo-fermentative production of d-lactic acid by Lactobacillus sp. employing casein whey permeate as a raw feed-stock. Biotechnol Lett 36(6):1303–1307

    Article  CAS  Google Scholar 

  • Renitha TS, Sridevi J, Gowthaman M, Kamini N, Ramanaiah B, Saravanan P (2015) Wealth from waste–beef extract for microbiological media from tannery solid waste. RSC Adv 5(13):9891–9897

    Article  Google Scholar 

  • Rezzadori K, Benedetti S, Amante ER (2012) Proposals for the residues recovery: Orange waste as raw material for new products. Food Bioprod Process 90(4):606–614

    Article  CAS  Google Scholar 

  • Ritala A, Häkkinen ST, Toivari M, Wiebe MG (2017) Single cell protein—state-of-the-art, industrial landscape and patents 2001–2016. Front Microbiol 8:2009

    Article  Google Scholar 

  • Singhvi M, Jadhav A, Gokhale D (2013) Supplementation of medium with diammonium hydrogen phosphate enhanced the D-lactate dehydrogenase levels leading to increased D-lactic acid productivity. Bioresour Technol 146:736–739

    Article  CAS  Google Scholar 

  • Singhvi M, Zendo T, Sonomoto K (2018) Free lactic acid production under acidic conditions by lactic acid bacteria strains: challenges and future prospects. Appl Microbiol Biotechnol 102(14):1–14

    Article  CAS  Google Scholar 

  • Tan J, Jahim J, Wu T, Harun S, Mumtaz T (2016) Use of corn steep liquor as an economical nitrogen source for biosuccinic acid production by Actinobacillus succinogenes. In: IOP Conference Series: Earth and Environmental Science, vol 36. IOP Publishing, p 012058

  • Tashiro Y, Kaneko W, Sun Y, Shibata K, Inokuma K, Zendo T, Sonomoto K (2011) Continuous d-lactic acid production by a novelthermotolerant Lactobacillus delbrueckii subsp. lactis QU 41. Appl Microbiol Biotechnol 89(6):1741–1750

    Article  CAS  Google Scholar 

  • Tejayadi S, Cheryan M (1995) Lactic acid from cheese whey permeate. Productivity and economics of a continuous membrane bioreactor. Appl Microbiol Biotechnol 43(2):242–248

    Article  CAS  Google Scholar 

  • Van Dyk JS, Gama R, Morrison D, Swart S, Pletschke BI (2013) Food processing waste: problems, current management and prospects for utilisation of the lignocellulose component through enzyme synergistic degradation. Renew Sust Energ Rev 26:521–531

    Article  Google Scholar 

  • Wang L, Zhao B, Li F, Xu K, Ma C, Tao F, Li Q, Xu P (2011) Highly efficient production of D-lactate by Sporolactobacillus sp. CASD with simultaneous enzymatic hydrolysis of peanut meal. Appl Microbiol Biotechnol 89(4):1009–1017

    Article  CAS  Google Scholar 

  • Wang Y, Tashiro Y, Sonomoto K (2015) Fermentative production of lactic acid from renewable materials: recent achievements, prospects, and limits. J Biosci Bioeng 119(1):10–18

    Article  CAS  Google Scholar 

  • Yoo I-K, Chang HN, Lee EG, Chang YK, Moon S-H (1997) Effect of B vitamin supplementation on lactic acid production by Lactobacillus casei. J Ferment Bioeng 84(2):172–175

    Article  CAS  Google Scholar 

  • Zhang Y, Vadlani PV (2013) D-Lactic acid biosynthesis from biomass-derived sugars via Lactobacillus delbrueckii fermentation. Bioprocess Biosyst Eng 36(12):1897–1904

    Article  CAS  Google Scholar 

  • Zhang Y, Vadlani PV, Kumar A, Hardwidge PR, Govind R, Tanaka T, Kondo A (2016) Enhanced D-lactic acid production from renewable resources using engineered Lactobacillus plantarum. Appl Microbiol Biotechnol 100(1):279–288

    Article  CAS  Google Scholar 

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Acknowledgments

The authors are grateful to Biopolis S.L. for supplying CSL from Roquette. They are as well grateful to Novozymes A/S for the kind gift of enzymes for hydrolysis of OPW.

Funding

This work has been funded by MINECO under contracts CTQ2013-45970-C2-1-R and PCIN-2013-021-C02-01 and BSCH-UCM, GR35/10-A 910134.

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Authors and Affiliations

  1. Materials and Chemical Engineering Department, Chemical Sciences School, Complutense University of Madrid, 28040, Madrid, Spain

    I. de la Torre, M. Ladero & V. E. Santos

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  1. I. de la Torre
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  2. M. Ladero
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  3. V. E. Santos
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Correspondence to M. Ladero.

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de la Torre, I., Ladero, M. & Santos, V.E. Production of d-lactic acid by Lactobacillus delbrueckii ssp. delbrueckii from orange peel waste: techno-economical assessment of nitrogen sources. Appl Microbiol Biotechnol 102, 10511–10521 (2018). https://doi.org/10.1007/s00253-018-9432-4

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  • DOI: https://doi.org/10.1007/s00253-018-9432-4

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