Temporal and spatial distribution of ammonia-oxidizing organisms of two types of wetlands in Northeast China
Ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) contribute significantly to the nitrogen cycle. The community structure of AOA and AOB is diverse in the different wetlands. Few studies have comparatively investigated the community structure and dynamics of ammonia-oxidizing organisms in the marsh wetland (Sanjiang wetland) and inland saline-alkaline wetland (Zhalong wetland) in Northeast China. In this study, soil samples were collected from two wetlands with different soil properties in July and October. The community structure of AOA and AOB based on the amoA gene was investigated by high throughput sequencing. The result showed that AOA affiliated to the Nitrososphaera lineage (1.1b group) were the dominant AOA in both Sanjiang and Zhalong wetlands, while AOB belonging to the Nitrosospira lineage was the dominant AOB in the Sanjiang wetland. AOB belonging to the Nitrosospira lineage and Nitrosomonas lineage were the dominant AOB in the Zhalong wetland in July and October, respectively. The dominant AOA and AOB in the Sanjiang wetland had no obvious variation from July to October, but the AOA and AOB communities in the Zhalong wetland changed a lot from July to October. Shannon and Simpson indexes showed the diversity of AOA in the Zhalong wetland was higher than that in the Sanjiang wetland, but the diversity of AOB in the Zhalong wetland was lower than that in the Sanjiang wetland. Nitrate (NO3−) and ammonium (NH4+) concentration and pH were the most significant factors influencing the community structure of AOA and AOB.
KeywordsAmmonia-oxidizing archaea Ammonia-oxidizing bacteria Wetland Genetic diversity amoA gene
This research was supported by National Natural Science Foundation of China (No. 31470543) and the State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (2014DX07).
Compliance with ethical standards
This article does not contain any studies with human participants or animals performed by any of the authors.
Conflict of interest
The authors declare that they have no conflict of interest.
- Chen CH, Gao DW, Tao Y (2013) Diversity and distribution of ammonia-oxidizing Archaea in the seasonally frozen soils in Northeastern China. Appl Environ Microbiol 97(14):6571–6579Google Scholar
- He JZ, Shen JP, Zhang LM, Zhu YG, Zheng YM, Xu MG, Di H (2007) Quantitative analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a Chinese upland red soil under long-term fertilization practices. Environ Microbiol 9(9):2364–2374CrossRefPubMedGoogle Scholar
- Pester M, Rattei T, Flechl S, Gröngröft A, Richter A, Overmann J, Reinhold-Hurek B, Loy A, Wagner M (2012) amoA-based consensus phylogeny of ammonia-oxidizing archaea and deep sequencing of amoA genes from soils of four different geographic regions. Environ Microbiol 14(2):525–539CrossRefPubMedPubMedCentralGoogle Scholar
- Zhang N, Zang SS (2015) Community structure characteristics of phytoplankton in Zhalong wetland, China. Pak J Bot 47(1):359–366Google Scholar
- Zhang X, Song C, Mao R, Yang G, Tao B, Shi F, Zhu X, Hou A (2014) Litter mass loss and nutrient dynamics of four emergent macrophytes during aerial decomposition in freshwater marshes of the Sanjiang plain, Northeast China. Plant Soil 385(1–2):139–147Google Scholar