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C-terminal Ser/Thr residues are vital for the regulatory role of Ste7 in the asexual cycle and virulence of Beauveria bassiana

  • Zhi-Kang Wang
  • Qing Cai
  • Sen-Miao Tong
  • Sheng-Hua Ying
  • Ming-Guang Feng
Biotechnologically relevant enzymes and proteins
  • 18 Downloads

Abstract

The mitogen-activated protein kinase (MAPK) kinase Ste7 has a conserved Ser/Thr loop (S/T-X4(6)-S/T) that can activate the MAPK Fus3 or Kss1 for the regulation of pheromone response and filamentous growth in model yeast. Here, we show that not only the loop but also four C-terminal Ser/Thr residues are essential for Ste7 to function in the Fus3 cascade of Beauveria bassiana, a filamentous fungal insect pathogen. Mutagenesis of either looped S216/T220 or C-terminal S362 resulted in the same severe defects in conidial germination, hyphal growth, aerial conidiation, and submerged blastospore production as the ste7 deletion, followed by a complete loss of virulence and similarly increased cell sensitivities to osmotic salts, oxidants, heat shock and UV-B irradiation. Mutagenesis of three other Ser/Thr residues (S391, S440, and T485) also caused severe defects in most of the mentioned phenotypes. These defects correlated well with dramatically reduced transcript levels of some phenotype-related genes. These genes encode a transcription factor (CreA) essential for carbon/nitrogen assimilation, developmental activators (BrlA, AbaA, and WetA) and upstream transcription factor (FluG) required for conidiation, P-type N+/K+ ATPases (Ena1–5) required for intracellular N+/K+ homeostasis, and antioxidant enzymes involved in multiple stress responses. Our study unveils that the loop and four C-terminal Ser/Thr residues are all vital for the regulatory role of Ste7 in the growth, conidiation, virulence, and/or stress tolerance of B. bassiana and perhaps other filamentous fungi.

Keywords

Entomopathogenic fungi MAPK cascade Fus3-cascaded component Phosphorylation sites Gene expression and regulation Asexual cycle Stress response Virulence 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 31572054), the Ministry of Science and Technology of the People’s Republic of China (Grant No. 2017YFD0201202), and the Fundamental Research Funds for the Central Universities (Grant No. 2018FZA6003).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Coastal EcologyLudong UniversityYantaiChina
  2. 2.Institute of Microbiology, College of Life SciencesZhejiang UniversityZhejiangChina
  3. 3.School of Agricultural and Food ScienceZhejiang A&F UniversityZhejiangChina

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