Mycobacterium tuberculosis toxin Rv2872 is an RNase involved in vancomycin stress response and biofilm development
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Bacterial toxin–antitoxin (TA) systems are emerging important regulators of multiple cellular physiological events and candidates for novel antibiotic targets. To explore the role of Mycobacterium tuberculosis function, unknown toxin gene Rv2872 was heterologously expressed in Mycobacterium smegmatis (MS_Rv2872). Upon induction, MS_Rv2872 phenotype differed significantly from the control, such as increased vancomycin resistance, retarded growth, cell wall, and biofilm structure. This phenotype change might result from the RNase activity of Rv2872 as purified Rv2872 toxin protein can cleave the products of several key genes involved in abovementioned phenotypes. In summary, toxin Rv2872 was firstly reported to be a endonuclease involved in antibiotic stress responses, cell wall structure, and biofilm development.
KeywordsToxin–antitoxin (TA) Antimicrobial resistance Biofilm RNase
We thank Professor Huang Hairong of Beijing Thoracic Hospital for providing the M. tuberculosis H37Rv genomic DNA and Professor Li Hongtao at School of Life Sciences, Southwest University, for the Western blot experiment support. This work was supported by National Natural Science Foundation (81371851, 81071316, 81271882, 81301394, 81172806, 81471563), National key R & D plan (2016YFC0502304), the Fundamental Research Funds for the Central Universities (XDJK2017D101, XDJK2017D100, XDJK2017D099) and Chongqing Municipal Education Science foundation (2015-JC-020).
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Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any other authors.
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