Abstract
The removal of harmful metals from the intestinal environment can be inhibited by various ions which can interfere with the adsorption of target metal ions. Therefore, it is important to understand the ion selectivity and adsorption mechanism of the adsorbent. In this study, we estimated the adsorption properties of Lactobacillus casei JCM1134 by analyzing the correlation between its maximum adsorption level (qmax) for seven metals and their ion characteristics. Some metal ions showed altered adsorption levels by L. casei JCM1134 as culture growth time increased. Although it was impossible to identify specific adsorption components, adsorption of Sr and Ba may depend on capsular polysaccharide levels. The maximum adsorption of L. casei JCM1134 (9 h of growth in culture) for divalent metal ions was in the following order: Cu2+ > Ba2+ > Sr2+ > Cd2+ > Co2+ > Mg2+ > Ni2+. The qmax showed a high positive correlation with the ionic radius. Because this tendency is similar to adsorption occurring through an ion exchange mechanism, it was inferred that an ion exchange mechanism contributed greatly to adsorption by L. casei JCM1134. Because the decrease in the amount of adsorption due to prolonged culture time was remarkable for metals with a large ion radius, it is likely that the adsorption components involved in the ion exchange mechanism decomposed over time. These results and analytical concept may be helpful for designing means to remove harmful metals from the intestinal tract.
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The manuscript has been edited carefully by a native-English-speaking professional editor from Editage, a division of Cactus Communications.
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R. E. was supported by a scholarship grant from the Futaba Electronics Memorial Foundation. This work was also supported in part by the Japan Society for the Promotion of Science KAKENHI Challenging Research (Exploratory) (grant number 17K19218) and The Sumitomo Electric Industries Group Corporate Social Responsibility Foundation (grant to H. A.).
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Endo, R., Aoyagi, H. Adsorption preference for divalent metal ions by Lactobacillus casei JCM1134. Appl Microbiol Biotechnol 102, 6155–6162 (2018). https://doi.org/10.1007/s00253-018-9050-1
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DOI: https://doi.org/10.1007/s00253-018-9050-1