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Applied Microbiology and Biotechnology

, Volume 102, Issue 12, pp 5299–5308 | Cite as

An effective established biosensor of bifunctional probes-labeled AuNPs combined with LAMP for detection of fish pathogen Streptococcus iniae

  • Ya Zhou
  • Jingfan Xiao
  • Xin Ma
  • Qiyao Wang
  • Yuanxing Zhang
Methods and protocols

Abstract

In purpose of valid Streptococcus iniae detection, we established a colorimetric biosensor using gold nanoparticles (AuNPs) labeled with dual functional probes and along with loop-mediated isothermal amplification (LAMP) assay (LAMP-AuNPs). Based on the characteristics of self-aggregation and bio-conjugation with ligands, AuNPs were chosen for observable color change in tandem with LAMP amplification method to reach high sensitivity and easy operation. Meanwhile, the improvement of dual probes that could fully utilize the LAMP product gave the biosensor a stable result exhibition. LAMP-AuNPs targeting gene ftsB, one of the ATP transporter-related genes, turned out favorable specificity in cross reaction among other fish pathogens. The detect limit of 102 CFU revealed a better sensitivity compared with polymerase chain reaction (PCR) method and AuNPs lateral flow test strip (LFTS). It was also proved to be effective by zebrafish infection model trials with less than 2-h time consumption and nearly no devices which make it a convenient biosensor for point-to-care S. iniae detection.

Keywords

LAMP Gold nanoparticles Bifunctional probes Streptococcus iniae 

Notes

Conflict of interest

The authors declare that they have no competing interests.

Funding

This work was supported by grants from the Ministry of Agriculture of China (nos. CARS-47-G17 and nyhyzx-201303047), the National Natural Sciences Foundation of China (no. 31672696), and the Project of Marine Public Welfare Profession (no. 201405003-2).

Compliance with ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiChina
  2. 2.Shanghai Engineering Research Center of Maricultured Animal VaccinesShanghaiChina
  3. 3.Shanghai Collaborative Innovation Center for BiomanufacturingShanghaiChina

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