A novel agaro-oligosaccharide-lytic β-galactosidase from Agarivorans gilvus WH0801
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β-Galactosidases have a wide application in the food and pharmaceutical industries. Recently, β-galactosidase was also found to participate in agar degradation. In this study, the second reported agarolytic β-galactosidase was found in the marine bacterium Agarivorans gilvus WH0801 and characterized. The β-galactosidase named AgWH2A (83 kDa) exhibited good activities under optimal hydrolysis conditions of pH 8.0 and 40 °C. AgWH2A could cleave the first D-galactose of agarooligosaccharides from its nonreducing end to produce neoagarooligosaccharides, but could not act on the neoagarooligosaccharides. AgWH2A has great potential in the comprehensive utilization of marine red algae.
Keywordsβ-Galactosidase GH2 family Agarivorans gilvus D-Galactose Agarooligosaccharides
This work was supported by the National Natural Science Foundation of China (31471607) and the Applied Basic Research Program of Qingdao (16-5-1-17-jch).
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Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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