High diversity of potential nitrate-reducing Fe(II)-oxidizing bacteria enriched from activated sludge
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Nitrate-dependent Fe(II) oxidation (NDFO) has been discovered in various environments including activated sludge and can potentially be used to remove nitrate from wastewater. In this study, NDFO sludge was successfully enriched from activated sludge under high Fe(II) concentrations over 100 days and the denitrification rate achieved 1.37 mmol N/(gVSS day). High-throughput sequencing of the bacterial 16S rRNA gene was used to investigate the microbial community structure dynamics during the enrichment process. The results showed that the microbial community changed significantly and high diversity of potential Fe(II)-oxidizing bacteria (FeOB) was observed in the enriched sludge. Thermomonas and Gallionella were the dominant bacterial genera in the enriched sludge and their relative abundances accounted for 9.49 and 4.08%, respectively. Furthermore, it was found that potential FeOB were also abundantly present in activated sludge samples of common municipal wastewater treatment plants. Collectively, this study demonstrated that NDFO could be successfully performed by enriched activated sludge and high diversity of bacteria is involved in this process, and the results also provide baseline information for future research and engineering application of NDFO process.
KeywordsNDFO Microbial community Fe(II)-oxidizing bacteria Activated sludge
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- APAH (2005) Standard methods for the examination of water and wastewater, 21st edn. American Public Health Association, Washington, DCGoogle Scholar
- Busse H, Kämpfer P, Moore E, Nuutinen J, Tsitko I, Denner E, Vauterin L, Valens M, Rosselló-Mora R, Salkinoja-Salonen M (2002) Thermomonas haemolytica gen. nov., sp. nov., a gamma-proteobacterium from kaolin slurry. Int J Syst Evol Microbiol 52(2):473–483. https://doi.org/10.1099/00207713-52-2-473 CrossRefPubMedGoogle Scholar
- Emerson D, Fleming EJ, McBeth JM (2010) Iron-oxidizing bacteria: an environmental and genomic perspective. Annu Rev Microbiol 64:561–583. https://doi.org/10.1146/annurev.micro.112408.134208 CrossRefPubMedGoogle Scholar
- Hallbeck L, Pedersen K (1991) Autotrophic and mixotrophic growth of Gallionella ferruginea. Microbiology 137(11):2657–2661Google Scholar
- Hauck S, Benz M, Brune A, Schink B (2001) Ferrous iron oxidation by denitrifying bacteria in profundal sediments of a deep lake (Lake Constance). FEMS Microbiol Ecol 37(2):127–134. https://doi.org/10.1111/j.1574-6941.2001.tb00860.x CrossRefGoogle Scholar
- Hegler F, Lösekann-Behrens T, Hanselmann K, Behrens S, Kappler A (2012) Influence of seasonal and geochemical changes on the geomicrobiology of an iron carbonate mineral water spring. Appl Environ Microbiol 78(20):7185–7196. https://doi.org/10.1128/AEM.01440-12 CrossRefPubMedPubMedCentralGoogle Scholar
- Klueglein N, Zeitvogel F, Stierhof Y-D, Floetenmeyer M, Konhauser KO, Kappler A, Obst M (2014) Potential role of nitrite for abiotic Fe(II) oxidation and cell encrustation during nitrate reduction by denitrifying bacteria. Appl Environ Microbiol 80(3):1051–1061. https://doi.org/10.1128/aem.03277-13 CrossRefPubMedPubMedCentralGoogle Scholar
- Mergaert J, Cnockaert MC, Swings J (2003) Thermomonas fusca sp. nov. and Thermomonas brevis sp. nov., two mesophilic species isolated from a denitrification reactor with poly (ε-caprolactone) plastic granules as fixed bed, and emended description of the genus Thermomonas. Int J Syst Evol Microbiol 53(6):1961–1966. https://doi.org/10.1099/ijs.0.02684-0 CrossRefPubMedGoogle Scholar
- Miot J, Maclellan K, Benzerara K, Boisset N (2011) Preservation of protein globules and peptidoglycan in the mineralized cell wall of nitrate-reducing, iron (II)-oxidizing bacteria: a cryo-electron microscopy study. Geobiology 9(6):459–470. https://doi.org/10.1111/j.1472-4669.2011.00298.x CrossRefPubMedGoogle Scholar
- Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75(23):7537–7541. https://doi.org/10.1128/AEM.01541-09 CrossRefPubMedPubMedCentralGoogle Scholar
- Wanner J (1994) Activated sludge: bulking and foaming control. CRC Press, Boca RatonGoogle Scholar
- Weber KA, Pollock J, Cole KA, O’Connor SM, Achenbach LA, Coates JD (2006) Anaerobic nitrate-dependent iron(II) bio-oxidation by a novel lithoautotrophic betaproteobacterium, strain 2002. Appl Environ Microbiol 72(1):686–694. https://doi.org/10.1128/aem.72.1.686-694.2006 CrossRefPubMedPubMedCentralGoogle Scholar
- Zhang M, Zheng P, Wang R, Li W, Lu H, Zhang J (2014) Nitrate-dependent anaerobic ferrous oxidation (NAFO) by denitrifying bacteria: a perspective autotrophic nitrogen pollution control technology. Chemosphere 117:604–609. https://doi.org/10.1016/j.chemosphere.2014.09.029 CrossRefPubMedGoogle Scholar
- Zhang M, Zheng P, Li W, Wang R, Ding S, Abbas G (2015) Performance of nitrate-dependent anaerobic ferrous oxidizing (NAFO) process: a novel prospective technology for autotrophic denitrification. Bioresour Technol 179:543–548. https://doi.org/10.1016/j.biortech.2014.12.036 CrossRefPubMedGoogle Scholar