Biotransformation using halotolerant yeast in seawater: a sustainable strategy to produce R-(−)-phenylacetylcarbinol
Acyloin condensation between benzaldehyde and decarboxylated pyruvate results in the production of R-(−)-phenylacetylcarbinol, a chiral precursor of the drug ephedrine. Huge research efforts have been made to improve the conditions of this reaction and to avoid the generation of by-products. Recently, we reported the advantages of using whole cells of the yeast Debaryomyces etchellsii as biocatalysts for this purpose. In this work, a new strategy, which fulfills green chemistry principles, is proposed and is based on using seawater as a gentle solvent. We demonstrate that, under these conditions, several improvements can be made compared to employing freshwater: (1) the conversion of the starting material in (R)-PAC is higher and with a minimum production of by-products; (2) it is possible to increase at least twofold the benzaldehyde load in the reaction medium; (3) cells can maintain their activity after several recycling rounds, which makes (R)-PAC production an easy and economical process.
KeywordsBiocatalysis Debaryomyces Green chemistry Phenylacetylcarbinol Seawater
This work was supported by the Universitat de València (UV-INV-AE15-323062). We gratefully acknowledge Dr. J. Ramos for providing us with the Debaryomyces hansenii strain CBS767 and the SCSIE (Universitat de València) for access to its instrumental facilities.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies conducted with animals performed by any of the authors.
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