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Applied Microbiology and Biotechnology

, Volume 102, Issue 11, pp 4717–4727 | Cite as

Biotransformation using halotolerant yeast in seawater: a sustainable strategy to produce R-(−)-phenylacetylcarbinol

  • Cecilia Andreu
  • Marcel ·lí del Olmo
Biotechnological products and process engineering

Abstract

Acyloin condensation between benzaldehyde and decarboxylated pyruvate results in the production of R-(−)-phenylacetylcarbinol, a chiral precursor of the drug ephedrine. Huge research efforts have been made to improve the conditions of this reaction and to avoid the generation of by-products. Recently, we reported the advantages of using whole cells of the yeast Debaryomyces etchellsii as biocatalysts for this purpose. In this work, a new strategy, which fulfills green chemistry principles, is proposed and is based on using seawater as a gentle solvent. We demonstrate that, under these conditions, several improvements can be made compared to employing freshwater: (1) the conversion of the starting material in (R)-PAC is higher and with a minimum production of by-products; (2) it is possible to increase at least twofold the benzaldehyde load in the reaction medium; (3) cells can maintain their activity after several recycling rounds, which makes (R)-PAC production an easy and economical process.

Keywords

Biocatalysis Debaryomyces Green chemistry Phenylacetylcarbinol Seawater 

Notes

Acknowledgements

This work was supported by the Universitat de València (UV-INV-AE15-323062). We gratefully acknowledge Dr. J. Ramos for providing us with the Debaryomyces hansenii strain CBS767 and the SCSIE (Universitat de València) for access to its instrumental facilities.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies conducted with animals performed by any of the authors.

Supplementary material

253_2018_8945_MOESM1_ESM.pdf (430 kb)
ESM 1 (PDF 429 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Departament de Química Orgànica, Facultat de FarmàciaUniversitat de València (UVEG)BurjassotSpain
  2. 2.Departament de Bioquímica i Biologia Molecular, Facultat de BiologiaUniversitat de València (UVEG)BurjassotSpain

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