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Applied Microbiology and Biotechnology

, Volume 102, Issue 10, pp 4393–4408 | Cite as

Multifunctional theranostic applications of biocompatible green-synthesized colloidal nanoparticles

  • Muhammad Ovais
  • Ali Talha Khalil
  • Abida Raza
  • Nazar Ul Islam
  • Muhammad Ayaz
  • Muthupandian Saravanan
  • Muhammad Ali
  • Irshad Ahmad
  • Muhammad Shahid
  • Zabta Khan Shinwari
Environmental biotechnology

Abstract

Phytochemicals offer immense promise for sustainable development and production of nanotechnology-enabled products. In the present study, Olax nana Wall. ex Benth. (family: Olacaceae) aqueous extract was used as an effective stabilizing agent to produce biogenic silver (ON-AgNPs) and gold nanoparticles (ON-AuNPs), which were investigated for biocompatibility and prospective biomedical applications (antibacterial, anticancer, antileishmanial, enzyme inhibition, antinociceptive, and anti-inflammatory activities). Various characterization techniques (XRD, FTIR, SEM, TEM, DLS, EDX, and SAED) revealed efficient biosynthesis of ON-AgNPs (26 nm) and ON-AuNPs (47 nm). In the toxicological assessment, ON-AgNPs and ON-AuNPs were found biocompatible towards human RBCs and macrophages (IC50 > 200 μg/mL). In a concentration range of 62.5–2000 μg/mL, a strong antibacterial effect was produced by ON-AgNPs against Staphylococcus epidermidis (MIC = 7.14 μg/mL) and Escherichia coli (8.25 μg/mL), while ON-AuNPs was only active against Staphylococcus aureus (9.14 μg/mL). At a concentration of 3.9–500 μg/mL, a dose-dependant inhibition of HepG2 cancer cells was produced by ON-AgNPs (IC50 = 14.93 μg/mL) and ON-AuNPs (2.97 μg/mL). Both ON-AgNPs and ON-AuNPs were found active against Leishmania tropica (KMH23) promastigotes (IC50 = 12.56 and 21.52 μg/mL) and amastigotes (17.44 and 42.20 μg/mL), respectively, after exposure to a concentration range of 1–200 μg/mL for 72 h. Preferential enzyme inhibition against urease and carbonic anhydrase II were noted for ON-AgNPs (39.23 and 8.89%) and ON-AuNPs (31.34 and 6.34%), respectively; however, these were found inactive against xanthine oxidase at 0.2 mg/mL. In the in vivo antinociceptive (acetic acid-induced abdominal constrictions) and anti-inflammatory (carrageenan-induced paw edema) activities, ON-AgNPs and ON-AuNPs at doses of 40 and 80 mg/kg, significantly attenuated the tonic nociception (P < 0.001) and ameliorated the carrageenan-induced inflammation (P < 0.01, P < 0.001). The results of in vitro and in vivo activities indicated that the biogenic nanoparticles can be used as valuable theranostic agents for further exploration of diverse biomedical applications.

Keywords

Phyto-nanotechnology Green synthesis of nanoparticles Green nanotechnology Biological potential of nanoparticles Nanoparticle drug delivery Biocompatible nanoparticles 

Notes

Acknowledgements

The authors highly acknowledge the Norwegian University of Science and Technology (NTNU), Norway for provision of TEM facility.

Funding

The study was funded by the PAK-NORWAY Institutional Cooperation Program, PK3004, and COMSTECH-TWAS project (12-198 RG/PHA/AS_C—UNESCO FR3240270874).

Compliance with ethical standards

Ethical approval

The in vivo biological activities were performed on BALB/c mice of either sex weighing 25–35 g. The animals were purchased from the National Institute of Health (NIH), Islamabad. These were acclimatized in a 12-h light/dark cycle at 22 ± 2 °C for 1 week prior to experiments. The animals had ad libitum access to food and water during this period. The experimental protocols on animals were approved by the Institutional Animals Use and Care Committee and were in accordance to the NIH guidelines for the care and use of laboratory animals.

Healthy adult male volunteers (ages ranging from 20 to 25 years) were recruited for the study and the inclusion was based on obtaining detailed medical history and clinical examination. The aims of the study were explained to the volunteers and informed consent was obtained from the respective participants at the start of the study. The study protocols were approved by the Institutional Ethical Committee and were in accordance with the principles of the 1964 Helsinki declaration and its later amendments.

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Ahamed M, Khan MM, Siddiqui M, AlSalhi MS, Alrokayan SA (2011) Green synthesis, characterization and evaluation of biocompatibility of silver nanoparticles. Physica E: Low Dimens Syst Nanostruct 43(6):1266–1271CrossRefGoogle Scholar
  2. Ahmed S, Ahmad M, Swami BL, Ikram S (2016) A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. Adv Res 7(1):17–28CrossRefGoogle Scholar
  3. Ahmmad B, Leonard K, Islam MS, Kurawaki J, Muruganandham M, Ohkubo T, Kuroda Y (2013) Green synthesis of mesoporous hematite (α-Fe 2 O 3) nanoparticles and their photocatalytic activity. Adv Powder Technol 24(1):160–167CrossRefGoogle Scholar
  4. Ai J, Biazar E, Jafarpour M, Montazeri M, Majdi A, Aminifard S, Zafari M, Akbari HR, Rad HG (2011) Nanotoxicology and nanoparticle safety in biomedical designs. Int J Nanomedicine 6:1117–1127PubMedPubMedCentralGoogle Scholar
  5. Ali A, Ambreen S, Javed R, Tabassum S, ul Haq I, Zia M (2017) ZnO nanostructure fabrication in different solvents transforms physio-chemical, biological and photodegradable properties. Mat Sci Eng C 74:137–145CrossRefGoogle Scholar
  6. Anwar A, Ovais M, Khan A, Raza A (2017) Docetaxel loaded solid lipid nanoparticles: a novel drug delivery system. IET Nanobiotechnol 11(6):621-629Google Scholar
  7. Arslan O (2001) Inhibition of bovine carbonic anhydrase by new sulfonamide compounds. Biochem Mosc 66(9):982–983CrossRefGoogle Scholar
  8. Ayaz M, Junaid M, Ullah F, Sadiq A, Ovais M, Ahmad W, Zeb A (2016) Chemical profiling, antimicrobial and insecticidal evaluations of Polygonum hydropiper L. BMC Compl Altern Med 16(1):502CrossRefGoogle Scholar
  9. Bhadra MP, Sreedhar B, Patra CR (2014) Potential theranostics application of bio-synthesized silver nanoparticles (4-in-1 system). Theranostics 4:316–335CrossRefPubMedPubMedCentralGoogle Scholar
  10. Bhattacharyya R, Medhi K, Borkataki S (2016) Plants used traditionally to treat malaria by tea-tribes in Nagaon district of Assam, India. Pleione 10(2):297–301Google Scholar
  11. Bonifácio BV, da Silva PB (2014) Nanotechnology-based drug delivery systems and herbal medicines: a review. Int J Nanomedicine 9:1–15CrossRefPubMedGoogle Scholar
  12. Borthakur A, Bhattacharyya S, Anbazhagan AN, Kumar A, Dudeja PK, Tobacman JK (2012) Prolongation of carrageenan-induced inflammation in human colonic epithelial cells by activation of an NFκB-BCL10 loop. Biochim Biophys Acta (BBA) - Mol Basis Dis 1822(8):1300–1307CrossRefGoogle Scholar
  13. Brigger I, Dubernet C, Couvreur P (2002) Nanoparticles in cancer therapy and diagnosis. Adv Drug Deliv Rev 54(5):631–651CrossRefPubMedGoogle Scholar
  14. Castro-Aceituno V, Ahn S, Simu SY, Singh P, Mathiyalagan R, Lee HA, Yang DC (2016) Anticancer activity of silver nanoparticles from Panax ginseng fresh leaves in human cancer cells. Biomed Pharmacother 84:158–165CrossRefPubMedGoogle Scholar
  15. Clares B, A Ruiz M, Gallardo V, L Arias J (2012) Drug delivery to inflammation based on nanoparticles surface decorated with biomolecules. Curr Med Chem 19(19):3203–3211CrossRefPubMedGoogle Scholar
  16. Collier H, Dinneen L, Johnson CA, Schneider C (1968) The abdominal constriction response and its suppression by analgesic drugs in the mouse. Br J Pharmacol Chemother 32(2):295–310CrossRefPubMedPubMedCentralGoogle Scholar
  17. Copeland RA (2013) Why enzymes as drug targets? In: Copeland RA (ed) Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists, 2nd edn. John Wiley & Sons, Inc., Hoboken, New Jersey, pp 1-23Google Scholar
  18. de Almeida MC, Silva AC, Barral A, Barral Netto M (2000) A simple method for human peripheral blood monocyte isolation. Mem Inst Oswaldo Cruz 95(2):221–223CrossRefPubMedGoogle Scholar
  19. De Jong WH, Borm PJ (2008) Drug delivery and nanoparticles: applications and hazards. Int J Nanomedicine 3(2):133–149CrossRefPubMedPubMedCentralGoogle Scholar
  20. Dizaj SM, Lotfipour F, Barzegar-Jalali M, Zarrintan MH, Adibkia K (2014) Antimicrobial activity of the metals and metal oxide nanoparticles. Mat Sci Eng C 44:278–284CrossRefGoogle Scholar
  21. Dobrovolskaia MA, Clogston JD, Neun BW, Hall JB, Patri AK, McNeil SE (2008) Method for analysis of nanoparticle hemolytic properties in vitro. Nano Lett 8(8):2180–2187CrossRefPubMedPubMedCentralGoogle Scholar
  22. El-Nour KMA, Eftaiha AA, Al-Warthan A, Ammar RA (2010) Synthesis and applications of silver nanoparticles. Arab J Chem 3(3):135–140CrossRefGoogle Scholar
  23. Fatima H, Khan K, Zia M, Ur-Rehman T, Mirza B, Haq I-u (2015) Extraction optimization of medicinally important metabolites from Datura innoxia Mill.: an in vitro biological and phytochemical investigation. BMC Compl Altern Med 15(1):376CrossRefGoogle Scholar
  24. Fedlheim DL, Foss CA (2001) Metal Nanoparticles: Synthesis, Characterization, and Applications. CRC Press, Boca Raton, FL, USAGoogle Scholar
  25. Fehrenbacher JC, Vasko MR, Duarte DB (2012) Models of inflammation: carrageenan-or complete Freund’s adjuvant (CFA)–induced edema and hypersensitivity in the rat. Curr Protoc Pharmacol 5.4:1–5.4. 4Google Scholar
  26. Ferrari M (2005) Cancer nanotechnology: opportunities and challenges. Nat Rev Cancer 5(3):161–171CrossRefPubMedGoogle Scholar
  27. FutureMarketInsights (2017a) Global market for metal & metal oxide nanoparticles to surge at more than 10% CAGR. PUblisher. http://markets.businessinsider.com/news/stocks/Global-Market-for-Metal-Metal-Oxide-Nanoparticles-to-Surge-at-More-Than-10-CAGR-1001862836 Accessed 9 May 2017
  28. FutureMarketInsights (2017b) Metal & metal oxide nanoparticles market: gold nanoparticles continue to shine in terms of value owing to significant market demand: global industry analysis and opportunity assessment, 2016-2026. PUblisher. http://www.futuremarketinsights.com/reports/metal-and-metal-oxide-nanoparticles-market. Accessed 9 May 2017
  29. Gray A, Spencer P, Sewell RDE (1998) The involvement of the opioidergic system in the antinociceptive mechanism of action of antidepressant compounds. Br J Pharmacol 124(4):669–674CrossRefPubMedPubMedCentralGoogle Scholar
  30. Gray AM, Pache DM, Sewell RD (1999) Do α 2-adrenoceptors play an integral role in the antinociceptive mechanism of action of antidepressant compounds? Eur J Pharmacol 378(2):161–168CrossRefPubMedGoogle Scholar
  31. Gupta AK, Gupta M (2005) Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 26(18):3995–4021CrossRefPubMedGoogle Scholar
  32. Gutiérrez V, Seabra AB, Reguera RM, Khandare J, Calderón M (2016) New approaches from nanomedicine for treating leishmaniasis. Chem Soc Rev 45(1):152–168CrossRefPubMedGoogle Scholar
  33. Islam NU, Ahsan F, Khan I, Shah MR, Shahid M, Khan MA (2015a) Green synthesis and biological activities of gold nanoparticles functionalized with Citrus reticulata, Citrus aurantium, Citrus sinensis and Citrus grandis. J Chem Soc Pak 37(4):721–731Google Scholar
  34. Islam NU, Jalil K, Shahid M, Muhammad N, Rauf A (2015b) Pistacia integerrima gall extract mediated green synthesis of gold nanoparticles and their biological activities. Arab J Chem.  https://doi.org/10.1016/j.arabjc.2015.02.014
  35. Islam NU, Jalil K, Shahid M, Rauf A, Muhammad N, Khan A, Shah MR, Khan MA (2015c) Green synthesis and biological activities of gold nanoparticles functionalized with Salix alba. Arab J Chem.  https://doi.org/10.1016/j.arabjc.2015.06.025
  36. Islam NU, Khan I, Rauf A, Muhammad N, Shahid M, Shah MR (2015d) Antinociceptive, muscle relaxant and sedative activities of gold nanoparticles generated by methanolic extract of Euphorbia milii. BMC Complement Altern Med 15(1):160CrossRefPubMedPubMedCentralGoogle Scholar
  37. Islam NU, Amin R, Shahid M, Amin M (2016) Gummy gold and silver nanoparticles of apricot (Prunus armeniaca) confer high stability and biological activity. Arab J Chem.  https://doi.org/10.1016/j.arabjc.2016.02.017
  38. Islam NU, Amin R, Shahid M, Amin M, Zaib S, Iqbal J (2017) A multi-target therapeutic potential of Prunus domestica gum stabilized nanoparticles exhibited prospective anticancer, antibacterial, urease-inhibition, anti-inflammatory and analgesic properties. BMC Complement Altern Med 17(1):276CrossRefPubMedPubMedCentralGoogle Scholar
  39. Jebali A, Kazemi B (2013) Nano-based antileishmanial agents: a toxicological study on nanoparticles for future treatment of cutaneous leishmaniasis. Toxicol in Vitro 27(6):1896–1904CrossRefPubMedGoogle Scholar
  40. Kah JCY (2013) Stability and aggregation assays of nanoparticles in biological media. In: Bergese P, Hamad-Schifferli K (eds) Nanomaterial Interfaces in Biology: Methods and Protocols Humana Press, Vol. 1025, pp 119-126. Google Scholar
  41. Kasithevar M, Saravanan M, Prakash P, Kumar H, Ovais M, Barabadi H, Shinwari ZK (2017) Green synthesis of silver nanoparticles using Alysicarpus monilifer leaf extract and its antibacterial activity against MRSA and CoNS isolates in HIV patients. J Interdiscip Nanomed 2(2):131–141CrossRefGoogle Scholar
  42. Kaye P, Scott P (2011) Leishmaniasis: complexity at the host-pathogen interface. Nat Rev Microbiol 9(8):604–616CrossRefPubMedGoogle Scholar
  43. Khalil AT, Ali M, Tanveer F, Ovais M, Idrees M, Shinwari ZK, Hollenbeck JE (2017a) Emerging viral infections in Pakistan: issues, concerns, and future prospects. Health Secur 15(3):268–281CrossRefPubMedGoogle Scholar
  44. Khalil AT, Ovais M, Ullah I, Ali M, Khan Shinwari Z, Maaza M (2017b) Biosynthesis of iron oxide (Fe2O3) nanoparticles via aqueous extracts of Sageretia thea (Osbeck.) and their pharmacognostic properties. Green Chem Lett Rev 10(4):186–201CrossRefGoogle Scholar
  45. Khalil AT, Ovais M, Ullah I, Ali M, Shinwari ZK, Hassan D, Maaza M (2017c) Sageretia thea (Osbeck.) modulated biosynthesis of NiO nanoparticles and their in vitro pharmacognostic, antioxidant and cytotoxic potential. Artif Cells Nanomed Biotechnol:1–15Google Scholar
  46. Khalil AT, Ovais M, Ullah I, Ali M, Shinwari ZK, Khamlich S, Maaza M (2017d) Sageretia thea (Osbeck.) mediated synthesis of zinc oxide nanoparticles and its biological applications. Nanomedicine 12(15):1767–1789CrossRefPubMedGoogle Scholar
  47. Khalil AT, Ovais M, Ullah I, Ali M, Shinwari ZK, Maaza M (2017e) Physical properties, biological applications and biocompatibility studies on biosynthesized single phase cobalt oxide (Co3O4) nanoparticles via Sageretia thea (Osbeck.) Arab J Chem.  https://doi.org/10.1016/j.arabjc.2017.07.004
  48. Lee SK, Mbwambo Z, Chung H, Luyengi L, Gamez E, Mehta R, Kinghorn A, Pezzuto J (1998) Evaluation of the antioxidant potential of natural products. Comb Chem High Throughput Screen 1(1):35–46PubMedGoogle Scholar
  49. Malagoli D (2007) A full-length protocol to test hemolytic activity of palytoxin on human erythrocytes. Invertebrate Surviv J 4(2):92–94Google Scholar
  50. Matsumoto H, Naraba H, Ueno A, Fujiyoshi T, Murakami M, Kudo I, Oh-ishi S (1998) Induction of cyclooxygenase-2 causes an enhancement of writhing response in mice. Eur J Pharmacol 352(1):47–52CrossRefPubMedGoogle Scholar
  51. Mazario J, Gaitan G, Herrero JF (2001) Cyclooxygenase-1 vs. cyclooxygenase-2 inhibitors in the induction of antinociception in rodent withdrawal reflexes. Neuropharmacology 40(7):937–946CrossRefPubMedGoogle Scholar
  52. Merskey H, Bogduk N (1994) Task Force on Taxonomy of the International Association for the Study of Pain. Classification of chronic pain: descriptions of chronic pain syndromes and definition of pain terms, Second edn. IASP Press, Seattle, pp 210–213Google Scholar
  53. Millan MJ (1999) The induction of pain: an integrative review. Prog Neurobiol 57(1):1–164CrossRefPubMedGoogle Scholar
  54. Mittal AK, Chisti Y, Banerjee UC (2013) Synthesis of metallic nanoparticles using plant extracts. Biotechnol Adv 31(2):346–356CrossRefPubMedGoogle Scholar
  55. Modolo LV, de Souza AX, Horta LP, Araujo DP, de Fátima  (2015) An overview on the potential of natural products as ureases inhibitors: a review. J Adv Res 6(1):35–44CrossRefPubMedGoogle Scholar
  56. Morens DM, Folkers GK, Fauci AS (2004) The challenge of emerging and re-emerging infectious diseases. Nature 430(6996):242–249CrossRefPubMedGoogle Scholar
  57. Morris CJ (2003) Carrageenan-induced paw edema in the rat and mouse. Inflamm Protoc (255):115–121Google Scholar
  58. Moulton MC, Braydich-Stolle LK, Nadagouda MN, Kunzelman S, Hussain SM, Varma RS (2010) Synthesis, characterization and biocompatibility of “green” synthesized silver nanoparticles using tea polyphenols. Nanoscale 2(5):763–770CrossRefPubMedGoogle Scholar
  59. Naahidi S, Jafari M, Edalat F, Raymond K, Khademhosseini A, Chen P (2013) Biocompatibility of engineered nanoparticles for drug delivery. J Control Release 166(2):182–194CrossRefPubMedGoogle Scholar
  60. Nathan C (2002) Points of control in inflammation. Nature 420(6917):846–852CrossRefPubMedGoogle Scholar
  61. Ovais M, Khalil AT, Raza A, Khan MA, Ahmad I, Islam NU, Saravanan M, Ubaid MF, Ali M, Shinwari ZK (2016) Green synthesis of silver nanoparticles via plant extracts: beginning a new era in cancer theranostics. Nanomedicine 12(23):3157–3177CrossRefGoogle Scholar
  62. Ovais M, Raza A, Naz S, Islam NU, Khalil AT, Ali S, Khan MA, Shinwari ZK (2017) Current state and prospects of the phytosynthesized colloidal gold nanoparticles and their applications in cancer theranostics. App Microbiol Biotechnol:1–15Google Scholar
  63. Ovais M, Ayaz M, Khalil AT, Shah SA, Jan MS, Raza A, Shahid M, Shinwari ZK (2018) HPLC-DAD finger printing, antioxidant, cholinesterase, and α-glucosidase inhibitory potentials of a novel plant Olax nana. BMC Compl Altern Med 18(1):1CrossRefGoogle Scholar
  64. Polk DB, Peek RM (2010) Helicobacter pylori: gastric cancer and beyond. Nat Rev Cancer 10(6):403–414CrossRefPubMedPubMedCentralGoogle Scholar
  65. Randhawa PK, Singh K, Singh N, Jaggi AS (2014) A review on chemical-induced inflammatory bowel disease models in rodents. Korean J Physiol Pharmacol 18(4):279–288CrossRefPubMedPubMedCentralGoogle Scholar
  66. Ren K, Dubner R (1999) Inflammatory models of pain and hyperalgesia. ILAR J 40(3):111–118CrossRefPubMedGoogle Scholar
  67. Rizzello L, Cingolani R, Pompa PP (2013) Nanotechnology tools for antibacterial materials. Nanomedicine 8(5):807–821CrossRefPubMedGoogle Scholar
  68. Saini D, Dubey R, Srivastava RJ, Singh KK, Chakraborti M (2010) Utilization of traditional plant diversity for poverty eradication in India. National Conference on Biodiversity, Development and Poverty Alleviation Uttar Pradesh State Biodiversity Board, pp 137-144Google Scholar
  69. Saravanan M, Vemu AK, Barik SK (2011) Rapid biosynthesis of silver nanoparticles from Bacillus megaterium (NCIM 2326) and their antibacterial activity on multi drug resistant clinical pathogens. Colloids Surf B Biointerfaces 88(1):325–331CrossRefPubMedGoogle Scholar
  70. Soh JH, Gao Z (2012) Metal nanoparticles in biomedical applications. In: Sau TK, Rogach AL (eds) Complex-shaped metal nanoparticles: bottom-up syntheses and applications. Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim, Germany, pp 477-519Google Scholar
  71. Song JY, Kim BS (2009) Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst Eng 32(1):79–84CrossRefPubMedGoogle Scholar
  72. Sperling RA, Gil PR, Zhang F, Zanella M, Parak WJ (2008) Biological applications of gold nanoparticles. Chem Soc Rev 37(9):1896–1908CrossRefPubMedGoogle Scholar
  73. Sprintz M, Benedetti C, Ferrari M (2004) Applied nanotechnology for the management of breakthrough cancer pain. Minerva Anestesiol 71(7–8):419–423Google Scholar
  74. Sprintz M, Tasciotti E, Allegri M, Grattoni A, Driver LC, Ferrari M (2011) Nanomedicine: ushering in a new era of pain management. Eur J Pain Suppl 5(S2):317–322CrossRefGoogle Scholar
  75. Subbaiya R, Priya A, Shankar K, Selvam M, Ovais M, Balajee R, Barabadi H, Muthupandian S (2017) Biomimetic synthesis of silver nanoparticles from Streptomyces atrovirens and their potential anticancer activity against human breast cancer cells. IET Nanobiotechnol 11(8):965-972Google Scholar
  76. Supuran CT (2008) Carbonic anhydrases: novel therapeutic applications for inhibitors and activators. Nat Rev Drug Discov 7(2):168–181CrossRefPubMedGoogle Scholar
  77. Thatoi P, Kerry RG, Gouda S, Das G, Pramanik K, Thatoi H, Patra JK (2016) Photo-mediated green synthesis of silver and zinc oxide nanoparticles using aqueous extracts of two mangrove plant species, Heritiera fomes and Sonneratia apetala and investigation of their biomedical applications. J Photochem Photobiol B Biol 163:311–318CrossRefGoogle Scholar
  78. Weatherburn M (1967) Phenol-hypochlorite reaction for determination of ammonia. Anal Chem 39(8):971–974CrossRefGoogle Scholar
  79. Wiegand I, Hilpert K, Hancock RE (2008) Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc 3(2):163–175CrossRefPubMedGoogle Scholar
  80. Woodford N, Livermore DM (2009) Infections caused by Gram-positive bacteria: a review of the global challenge. J Inf Secur 59:S4–S16Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Muhammad Ovais
    • 1
    • 2
    • 3
  • Ali Talha Khalil
    • 1
    • 4
  • Abida Raza
    • 2
  • Nazar Ul Islam
    • 5
  • Muhammad Ayaz
    • 6
  • Muthupandian Saravanan
    • 7
  • Muhammad Ali
    • 1
  • Irshad Ahmad
    • 8
  • Muhammad Shahid
    • 5
  • Zabta Khan Shinwari
    • 1
    • 4
    • 9
  1. 1.Department of BiotechnologyQuaid-i-Azam UniversityIslamabadPakistan
  2. 2.Pakistan Atomic Energy CommissionNational Institute for Lasers and Optronics (NILOP)IslamabadPakistan
  3. 3.Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijingChina
  4. 4.Department of Eastern Medicine and SurgeryQarshi UniversityLahorePakistan
  5. 5.Department of PharmacySarhad University of Science and Information TechnologyPeshawarPakistan
  6. 6.Department of PharmacyUniversity of MalakandChakdaraPakistan
  7. 7.Department of Medical Microbiology and Immunology, Institute of Biomedical Sciences, College of Health SciencesMekelle UniversityMekelleEthiopia
  8. 8.Department of Life SciencesKing Fahd University of Petroleum and MineralsDhahranSaudi Arabia
  9. 9.Pakistan Academy of SciencesIslamabadPakistan

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