Heat and light stresses affect metabolite production in the fruit body of the medicinal mushroom Cordyceps militaris
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Cordyceps militaris is a highly valued edible and medicinal fungus due to its production of various metabolites, including adenosine, cordycepin, N6-(2-hydroxyethyl)-adenosine, and carotenoids. The contents of these metabolites are indicative of the quality of commercially available fruit body of this fungus. In this work, the effects of environmental abiotic factors, including heat and light stresses, on the fruit body growth and metabolite production in C. militaris were evaluated during the late growth stage. The optimal growth temperature of C. militaris was 20 °C. It was found that a heat stress of 25 °C for 5–20 days during the late growth stage significantly promoted cordycepin and carotenoid production without affecting the biological efficiency. Light stress at 6000 lx for 5–20 days during the late growth stage significantly promoted cordycepin production but decreased the carotenoid content. Both heat and light stresses promoted N6-(2-hydroxyethyl)-adenosine production. In addition, gene expression analysis showed that there were simultaneous increases in the expression of genes encoding a metal-dependent phosphohydrolase (CCM_04437) and ATP phosphoribosyltransferase (CCM_04438) that are involved in the cordycepin biosynthesis pathway, which was consistent with the accumulation of cordycepin during heat stress for 5–20 days. A positive weak correlation between the cordycepin and adenosine contents was observed with a Pearson correlation coefficient of 0.338 (P < 0.05). The results presented herein provide a new strategy for the production of a superior quality fruit body of C. militaris and contribute to further elucidation of the effects of abiotic stress on metabolite accumulation in fungi.
KeywordsCordyceps militaris Cordycepin Carotenoid Heat stress Light stress
This study was funded by the National Natural Science Foundation of China (31572179, 31600054), the Coal-based Key Scientific and Technological Project from Shanxi Province (FT2014-03-01), and the Key Research and Development Program from Guangxi Province (2016AB05317).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Chai YQ, Wei ZM, Chen ZA, Li XL, Liu YG, Wang GE (2004) N6-(2-hydroxyethyl)-adenosine’ application in the preparation of analgesic drugs. China, ZL200410094511.0. (in Chinese)Google Scholar
- Chang ST, Miles PG (2004) Mushrooms: cultivation, nutritional value, medicinal effect and environmental impact. CRC Press LLC 38(4):688–692Google Scholar
- Chen C, Bau T, Bao HY (2013) Chemical composition analysis of cultured Cordyceps militaris. Food Sci 34(11):36–40 (in Chinese)Google Scholar
- Fu MJ, Wang XJ (2005) Accumulation of carotenoid in Colletotrichum gloeosporioides induced by blue light. Acta Microbiol Sin 45(5):795–797Google Scholar
- OncoVista Inc. (2008) A phase I/II study of cordycepin plus pentostatin in patients with refractory TdT-positive leukemia. Clinical Trials. https://clinicaltrials.gov/show/NCT00709215. Accessed 2 Jan 2018
- Kornerup A, Wanscher JH (1978) Methuen handbook of colour. EyreMethuen, LondonGoogle Scholar
- Krupodorova TA, Barshteyn VY (2015) Alternative substrates for higher mushrooms mycelia cultivation. J Biosci Bioeng 4(3):339–347Google Scholar
- Lim LT, Lee CY, Chang ET (2012) Optimization of solid state culture conditions for the production of adenosine, cordycepin, and D-mannitol in fruiting bodies of medicinal caterpillar fungus Cordyceps militaris (L.:Fr.) Link (Ascomycetes). Int J Med Mushrooms 14(2):181–187CrossRefPubMedGoogle Scholar
- Razavi SH, Marc I (2006) Effect of temperature and pH on the growth kinetics and carotenoid production by Sporobolomyces ruberrimus H110 using technical glycerol as carbon source. Iran J Chem Chem Eng 23(3):59–64Google Scholar
- Yan XT, Bao HY, Bau T (2010) Isolation and identification of one natural pigment from cultured Cordyceps militaris. Mycosystema 29(5):777–781Google Scholar
- Zhang X, Ren A, Li MJ, Cao PF, Chen TX, Zhang G, Shi L, Jang AL, Zhao MW (2016) Heat stress modulates mycelium growth, heat shock protein expression, ganoderic acid biosynthesis and hyphal branching of Ganoderma lucidum via cytosolic Ca2+. Appl Environ Microbiol 82(14):4112–4125CrossRefPubMedPubMedCentralGoogle Scholar