Applied Microbiology and Biotechnology

, Volume 102, Issue 9, pp 3915–3937 | Cite as

Metabolic engineering of Corynebacterium glutamicum for fermentative production of chemicals in biorefinery

  • Kei-Anne Baritugo
  • Hee Taek Kim
  • Yokimiko David
  • Jong-il Choi
  • Soon Ho Hong
  • Ki Jun Jeong
  • Jong Hyun Choi
  • Jeong Chan Joo
  • Si Jae Park


Bio-based production of industrially important chemicals provides an eco-friendly alternative to current petrochemical-based processes. Because of the limited supply of fossil fuel reserves, various technologies utilizing microbial host strains for the sustainable production of platform chemicals from renewable biomass have been developed. Corynebacterium glutamicum is a non-pathogenic industrial microbial species traditionally used for l-glutamate and l-lysine production. It is a promising species for industrial production of bio-based chemicals because of its flexible metabolism that allows the utilization of a broad spectrum of carbon sources and the production of various amino acids. Classical breeding, systems, synthetic biology, and metabolic engineering approaches have been used to improve its applications, ranging from traditional amino-acid production to modern biorefinery systems for production of value-added platform chemicals. This review describes recent advances in the development of genetic engineering tools and techniques for the establishment and optimization of metabolic pathways for bio-based production of major C2–C6 platform chemicals using recombinant C. glutamicum.


Metabolic engineering Microbial cell factory Corynebacterium glutamicum Plasmid vectors Promoters Bio-based chemicals 



This work was supported by the Mid-career Researcher Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Science and ICT (MSIT) (NRF-2016R1A2B4008707), a basic research grant from the KRIBB, and the Lignin Biorefinery from MSIT through the NRF of Korea (NRF-2017M1A2A2087634).

Availability of data and material

Please contact corresponding author for any data requests.


Funding sources are declared in acknowledgement section.

Compliance with ethical standards

Competing interest

The authors declare that they have no competing interests.

Ethics approval and consent to participate

Not applicable. This article does not contain any studies with human participants or animals performed by any of the authors.

Consent for publication

Not applicable. Our manuscript does not contain any individual person’s data in any form.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Division of Chemical Engineering and Materials ScienceEwha Womans UniversitySeoulRepublic of Korea
  2. 2.Center for Bio-based Chemistry, Division of Convergence ChemistryKorea Research Institute of Chemical TechnologyDaejeonRepublic of Korea
  3. 3.Department of Biotechnology and BioengineeringChonnam National UniversityGwangjuRepublic of Korea
  4. 4.Department of Chemical EngineeringUniversity of UlsanUlsanRepublic of Korea
  5. 5.Department of Chemical and Biomolecular Engineering (BK21 Plus Program)Korea Advanced Institute of Science and Technology (KAIST)DaejeonRepublic of Korea
  6. 6.Microbial Biotechnology Research CenterJeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB)JeongeupRepublic of Korea

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