Intestinal bacterial signatures of white feces syndrome in shrimp
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Increasing evidence suggests that the intestinal microbiota is closely correlated with the host’s health status. Thus, a serious disturbance that disrupts the stability of the intestinal microecosystem could cause host disease. Shrimps are one of the most important products among fishery trading commodities. However, digestive system diseases, such as white feces syndrome (WFS), frequently occur in shrimp culture and have led to enormous economic losses across the world. The WFS occurrences are unclear. Here, we compared intestinal bacterial communities of WFS shrimp and healthy shrimp. Intestinal bacterial communities of WFS shrimp exhibited less diversity but were more heterogeneous than those of healthy shrimp. The intestinal bacterial communities were significantly different between WFS shrimp and healthy shrimp; compared with healthy shrimp, in WFS shrimp, Candidatus Bacilloplasma and Phascolarctobacterium were overrepresented, whereas Paracoccus and Lactococcus were underrepresented. PICRUSt functional predictions indicated that the relative abundances of genes involved in energy metabolism and genetic information processing were significantly greater in WFS shrimp. Collectively, we found that the composition and predicted functions of the intestinal bacterial community were markedly shifted by WFS. Significant increases in Candidatus Bacilloplasma and Phascolarctobacterium and decreases in Paracoccus and Lactococcus may contribute to WFS in shrimp.
KeywordsIntestinal microbiota Shrimp Pacific white shrimp White feces syndrome Diversity
This work was financially supported by the China Agriculture Research System (CARS-48), the Guangzhou Science Technology and Innovation Commission Project (201510010071), and the Guangdong Ocean and Fishery Bureau Project (20164200042090023).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
This article does not contain any studies with human participants by any of the authors. No specific permits were required for the described field studies. No specific permissions were required for access to the artificial pond in Maoming, Guangdong Province, China. The field studies did not involve endangered or protected species. This study was reviewed and approved by the ethics committee of Sun Yat-sen University.
- Clarke SF, Murphy EF, O,Sullivan O, Lucey AJ, Humphreys M, Hogan A (2014) Exercise and associated dietary extremes impact on gut microbial diversity. Gut 63: 1913–1920Google Scholar
- FAO (2015) Fishery and aquaculture statistics. Food and Agriculture Organization of the United Nations, RomeGoogle Scholar
- Langille MG, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes JA, Clemente JC, Burkepile DE, Thurber RLV, Knight R, Beiko RG, Huttenhower G (2013) Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat Biotechnol 31:814–821CrossRefPubMedPubMedCentralGoogle Scholar
- Sriurairatana S, Boonyawiwat V, Gangnonngiw W, Laosutthipong C, Hiranchan J, Flegel TW (2014) White feces syndrome of shrimp arises from transformation, sloughing and aggregation of hepatopancreatic microvilli into vermiform bodies superficially resembling gregarines. PLoS One 9(6):e99170CrossRefPubMedPubMedCentralGoogle Scholar
- Tangprasittipap A, Srisala J, Chouwdee S, Somboon M, Chuchird N, Limsuwan C, Srisuvan T, Flegel TW, Sritunyalucksana K (2013) The microsporidian Enterocytozoon hepatopenaei is not the cause of white feces syndrome in whiteleg shrimp Penaeus (Litopenaeus) vannamei. BMC Vet Res 9:139CrossRefPubMedPubMedCentralGoogle Scholar