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Applied Microbiology and Biotechnology

, Volume 102, Issue 7, pp 3159–3171 | Cite as

Aldopentoses as new substrates for the membrane-bound, pyrroloquinoline quinone-dependent glycerol (polyol) dehydrogenase of Gluconobacter sp.

  • Toshiharu Yakushi
  • Yuka Terada
  • Seishiro Ozaki
  • Naoya Kataoka
  • Yoshihiko Akakabe
  • Osao Adachi
  • Minenosuke Matsutani
  • Kazunobu Matsushita
Biotechnologically relevant enzymes and proteins

Abstract

Membrane-bound, pyrroloquinoline quinone (PQQ)-dependent glycerol dehydrogenase (GLDH, or polyol dehydrogenase) of Gluconobacter sp. oxidizes various secondary alcohols to produce the corresponding ketones, such as oxidation of D-sorbitol to L-sorbose in vitamin C production. Substrate specificity of GLDH is considered limited to secondary alcohols in the D-erythro configuration at the next to the last carbon. Here, we suggest that L-ribose, D- and L-lyxoses, and L-tagatose are also substrates of GLDH, but these sugars do not meet the substrate specificity rule of GLDH. The oxygen consumption activity of wild-type Gluconobacter frateurii cell membranes depends on several kinds of sugars as compared with that of the membranes of a GLDH-negative variant. Biotransformation of those sugars with the membranes was examined to determine the reaction products. A time course measuring the pH in the reaction mixture and the increase or decrease in substrates and products on TLC suggested that oxidation products of L-lyxose and L-tagatose were ketones with unknown structures, but those of L-ribose and D-lyxose were acids. The oxidation product of L-ribose was purified and revealed to be L-ribonate by HRMS and NMR analysis. Biotransformation of L-ribose with the membranes and also with the whole cells produced L-ribonate in nearly stoichiometric amounts, indicating that the specific oxidation site in L-ribose is recognized by GLDH. Since purified GLDH produced L-ribonate without any intermediate-like compounds, we propose here a reaction model where the first carbon in the pyranose form of L-ribose is oxidized by GLDH to L-ribonolactone, which is further hydrolyzed spontaneously to produce L-ribonate.

Keywords

Acetic acid bacteria Oxidative biotransformation Gluconobacter L-ribonic acid L-ribose 

Notes

Acknowledgements

We are grateful to Armin Ehrenreich (Technische Universität München, Germany) for kindly providing pKOS6b to us. We thank Yuka Narita, Takahiro Torikai, and Koichi Furuya (Yamaguchi University, Japan) for their technical assistances.

Funding

This study was funded by MEXT KAKENHI (grant numbers 17K07722 to TY; 2660068 to KM).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2018_8848_MOESM1_ESM.pdf (929 kb)
ESM 1 (PDF 929 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Division of Agricultural Science, Graduate School of Science and Technology for InnovationYamaguchi UniversityYamaguchiJapan
  2. 2.Department of Biological Chemistry, Faculty of AgricultureYamaguchi UniversityYamaguchiJapan
  3. 3.Research Center for Thermotolerant Microbial ResourcesYamaguchi UniversityYamaguchiJapan

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