Applied Microbiology and Biotechnology

, Volume 102, Issue 5, pp 2289–2299 | Cite as

Time-course responses of ileal and fecal microbiota and metabolite profiles to antibiotics in cannulated pigs

Applied microbial and cell physiology

Abstract

We investigated the time-course effects of therapeutic antibiotics on intestinal microbial composition and metabolism in an ileal-cannulated pig model. Sixteen ileal-cannulated piglets (12 ± 0.5 kg) were assigned to two groups (n = 8) and fed standard diets with or without antibiotics. At 4 days before, and at days 2, 7, and 13 after antibiotic administration, ileal and fecal samples were collected for analysis of microbiota composition via 16S rRNA MiSeq sequencing and metabolites (short-chain fatty acids, biogenic amines, and indole). It was found that Lactobacillus and Bifidobacterium had decreased by an average 2.68-fold and 508-fold in ileum on days 2–13, and by an average 45.08-fold and 71.50-fold in feces on days 7–13 (P < 0.05). Escherichia/Shigella had increased by an average 265-fold in ileum on days 2–13, and by an average 36.70-fold in feces on days 7–13 (P < 0.05). Acetate concentration had decreased in ileum by an average 2.88-fold on days 2–13, and by 1.83-fold in feces on day 7 (P < 0.05). Cadaverine concentration had increased by an average 7.03-fold in ileum on days 2–13, and by an average 9.96-fold in feces on days 7–13 (P < 0.05), and fecal indole concentration had increased by an average 2.51-fold on days 7–13 (P < 0.05). Correlation analysis between significant microbes and metabolites indicated that the antibiotic-induced microbiota shift appeared to result in the changes of intestinal metabolism. In conclusion, antibiotic administration led to dynamic changes in microbial communities and metabolism in ileum and feces, with ileal microbiota being more prone to shift than fecal microbiota.

Keywords

Antibiotics Dynamic impact of antibiotics Temporal change of gut microbiota Microbial metabolites 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Supplementary material

253_2018_8774_MOESM1_ESM.pdf (837 kb)
ESM 1 (PDF 837 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Kan Gao
    • 1
  • Yu Pi
    • 1
  • Yu Peng
    • 1
  • Chun-Long Mu
    • 1
  • Wei-Yun Zhu
    • 1
  1. 1.Laboratory of Gastrointestinal Microbiology, College of Animal Science and TechnologyNanjing Agricultural UniversityNanjingPeople’s Republic of China

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