Lead-resistant bacteria from Saint Clair River sediments and Pb removal in aqueous solutions
- 247 Downloads
Lead is an extensive contaminant. Pb-resistant bacterial strains were isolated from Saint Clair River sediments on two enrichment media with increasing concentrations of Pb (NO3)2. Bacterial strains that grew at 1.25 or 1.5 g L−1 of Pb (NO3)2 L−1) were purified and selected for further study. Ninety-seven Pb-resistant strains were screened for the ability to produce bioflocculants. The majority of the Pb-resistant strains demonstrated moderate to high flocculation activity. Metal removal assays demonstrated that the higher is the flocculation activity, the higher is the efficiency of metal removal. In the multi-metal solutions, the bacterial strain with the highest flocculation activity (R19) had the highest metal removing capability (six out of eight metals) and the highest metal removal efficiency. The highly selective affinity towards Pb2+ observed for strain R19 suggests its use for the recovery of Pb2+ from multiple metal solutions. Because they are well adapted to unfavorable conditions due to their resistance to metals (e.g., Pb) and antibiotics, these characteristics may help in developing an effective process for wastewater treatment using these strains.
KeywordsBioflocculant Biosorption Pb tolerance Exopolysaccharides Metal resistance
This research was funded by the University of Michigan-Dearborn Office of Sponsored Programs. We thanked the anonymous reviewers for critically reading the manuscript and suggesting substantial improvements.
Tiquia-Arashiro conceived and designed the study. Bowman performed the experiments and analyzed the data with Patel, Sanchez, Xu, and Alsaffar. Alsaffar collected the sediment samples and field data. Tiquia-Arashiro, Bowman, Patel, and Sanchez wrote the manuscript, which was completed with input from Xu and Alsaffar
Compliance with ethical standards
This article does not contain any experiments involving human participants or animals performed by any of the authors.
Conflict of interest
The authors declare that they have no conflict of interest.
- Cleveland LM, Minter ML, Cobb KA, Scott AA, German VF (2008) Lead hazards for pregnant women and children: part 1: immigrants and the poor shoulder most of the burden of lead exposure in this country. Am J Nurs 108:40–49Google Scholar
- Difco and BBL (2009) Difco and BBL manual of microbiological media, 2nd edn. BD Diagnostics-Diagnostic System, Sparks 700 pGoogle Scholar
- El-Hendawy HH, Ali Dena A, El-Shatoury EH, Ghanem SM (2009) Bioaccumulation of heavy metals by Vibrio alginolyticus isolated from wastes of iron and steel factory, Helwan, Egypt. Egypt Acad J Biol Sci 1:23–28Google Scholar
- Fogarty LR (2007) Bacteria and emerging chemical contaminants in the St. Clair River/Lake St. Clair Basin, Michigan: U.S. Geological Survey Open-File Report 2007–1083, 10Google Scholar
- Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Kreig NR, Phillips GB. Manual of Methods for General Bacteriology (1994) Washington (DC): American Society for Microbiology. 791 pGoogle Scholar
- Lin J, Harichund C (2011) Isolation and characterization of heavy metal removing bacterial bioflocculants. Afr J Microbiol Res 5:559–607Google Scholar
- Murthy S, Bali G, Sarangi SK (2012) Biosorption of lead by Bacillus cereus isolated from industrial effluents. British Biotechnology Journal 2:73–84Google Scholar
- Oest A, Alsaffar A, Fenner M, Azzopardi D, Tiquia-Arashiro SM (2018) Patterns of change in metabolic capabilities of sediment microbial communities along pollution gradients in river and lake ecosystems. Environmental Technology. In reviewGoogle Scholar
- Patel D, Gismondi R, Alsaffar A, Tiquia-Arashiro SM (2018) Extracellular enzyme activities in sediments of St. Clair River and Lake Saint Clair, Michigan. Journal of Microbiology. In reviewGoogle Scholar
- Summers AO, Wireman J, Vimy MJ, Lorscheider FL, Marshall B, Levy SB, Bennett S, Billard L (1993) Mercury released from dental silver fillings provokes an increase in mercury-resistant and antibiotic resistant bacteria in oral and intestinal floras of primates. Antimicrob Agents Chemother 37(4):825–834. https://doi.org/10.1128/AAC.37.4.825 CrossRefPubMedPubMedCentralGoogle Scholar