Immunological features and efficacy of the recombinant subunit vaccine LTB-EMY162 against Echinococcus multilocularis metacestode
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Alveolar echinococcosis is a zoonotic disease caused by the infection of the larval stage Echinococcus multilocularis with worldwide distribution especially in the northwest China. It is important to develop a well-tolerated immunoprophylaxis against E. multilocularis for alveolar echinococcosis control. In this study, a prokaryotic expression system for recombinant immunogen LTB-EMY162 was established, and the immunological features, sensitized lymphocyte, IL-4/IFN-γ secreted, prophylactic effect, and therapeutic effect were also evaluated. Arctic Express (DE3) system, Ni2+-charged and molecular sieve chromatography were used to obtain a high-purity 29 kDa protein. The ELISA and lymphocyte proliferation assay showed that LTB-EMY162 induced high-titer specific IgG against EMY162 and E. multilocularis protoscoleces protein in BALB/c mice and promoted sensitized T lymphocyte cell proliferation, and LTB-EMY162 stimulated Th cell to secrete IL-4 and IFN-γ and induced a Th1/Th2 mixed type immunological response. We also found that LTB-EMY162 significantly inhibited the cysts formation by challenging with 1000 E. multilocularis protoscoleces. The growth of protoscoleces and cysts were also significantly decreased by treating with LTB-EMY162 in 1000 protoscoleces intraperitoneal injection therapeutic mice model. In conclusion, we have constructed a subunit vaccine LTB-EMY162 which has prevention and therapeutic effect against E. multilocularis infection.
KeywordsEchinococcus multilocularis Alveolar echinococcosis Subunit vaccine EMY162
We thank all of the staffs for their helpful suggestions and advice during this project. We would like to thank the editor for the helpful comments on the manuscript.
This work was funded by National Natural Science Foundation of China (No.81360300, Tang F; No. 32571231, Ge RL), the Province Natural Science Foundation of Qinghai (No. 2015-ZJ-745), the 123Talent Project of Qinghai University, the Key Project of Qinghai Provincial Health and Family Planning Commission (No. 2016-wjzd-04), the National Basic Research Program of China (No. 2012CB518200, L-RG), and the Science and technology project of Qinghai Agriculture and Animal Husbandry Bureau (NMSY-2017-09). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
All experiments were conducted with adherence to the NIH Guide for the Care and Use of Laboratory Animals, and the protocol was approved by the Animal Ethical and Experimental Committee of Qinghai University (QHDX-2014-07). This article does not contain any studies with human participants performed by any of the authors.
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