Applied Microbiology and Biotechnology

, Volume 102, Issue 5, pp 2399–2412 | Cite as

The role of soil bacterial community during winter fallow period in the incidence of tobacco bacterial wilt disease

  • Yunhua Xiao
  • Xueduan Liu
  • Delong Meng
  • Jiemeng Tao
  • Yabing Gu
  • Huaqun Yin
  • Juan Li
Environmental biotechnology


Bacterial wilt, caused by Ralstonia solanacearum, occurs occasionally during tobacco planting and potentially brings huge economic losses in affected areas. Soil microbes in different management stages play important roles in influencing bacterial wilt incidence. Studies have focused on the impacts of species diversity and composition during cropping periods on disease morbidity; however, the effects of the soil bacterial biomass, species diversity, species succession, and population interactions on morbidity remain unclear during non-cropping periods. In this study, we explored the soil bacterial communities in the non-cropping winter fallow (WF) and cropping late growing (LG) periods under consecutive monoculture systems using 16S ribosomal RNA gene sequencing and qPCR and further analyzed their effects on tobacco bacterial wilt incidence. We found that the bacterial communities in the WF period were significantly different from those in the LG period based on detrended correspondence analysis and dissimilarity tests. Crop morbidity was significantly related to bacterial community structure and to the presence of some genera during WF and LG periods. These genera, such as Arthrobacter, Pseudomonas, Acidobacteria GP6, and Pasteuria, may be potential biological control agents for bacterial wilt. Further analysis indicated that low soil bacterial diversity during the WF period, decrease of bacterial interactions from the WF to LG periods, and low soil biomass during the LG period all have the potential to increase morbidity. In conclusion, an increase of soil bacterial diversity and control of some bacterial abundances in the WF period might be an effective approach in controlling bacterial wilt incidence.


Crop morbidity Biological control agents Bacterial interactions Bacterial diversity Bacterial community structure 


Author contribution

H. Y. and J. L. conceived of the experiment. Y. X. and H. Y. performed the experiment. Y. X. analyzed the data and wrote the manuscript. X. L., D. M., J. T., and Y. G. participated in the discussions.

Funding information

The study was supported by Key Project of Science and Technology of Hunan Branch of China National Tobacco Corporation (16-19Aa02) and the Graduate Student Research Innovation Project in Central South University (No. 2016zzts105).

Compliance with ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare that they have no conflict of interests.

Supplementary material

253_2018_8757_MOESM1_ESM.pdf (2.5 mb)
ESM 1 (PDF 2523 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Bioscience and Biotechnology and College of AgronomyHunan Agricultural UniversityChangshaChina
  2. 2.School of Minerals Processing and BioengineeringCentral South UniversityChangshaChina

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