Applied Microbiology and Biotechnology

, Volume 102, Issue 5, pp 2465–2475 | Cite as

Hydrogen metabolic patterns driven by Clostridium-Streptococcus community shifts in a continuous stirred tank reactor

  • Rodolfo Palomo-Briones
  • Eric Trably
  • Nguyen Esmeralda López-Lozano
  • Lourdes B. Celis
  • Hugo Oscar Méndez-Acosta
  • Nicolas Bernet
  • Elías Razo-Flores
Bioenergy and biofuels

Abstract

The hydrogen (H2) production efficiency in dark fermentation systems is strongly dependent on the occurrence of metabolic pathways derived from the selection of microbial species that either consume molecular H2 or outcompete hydrogenogenic bacteria for the organic substrate. In this study, the effect of organic loading rate (OLR) on the H2 production performance, the metabolic pathways, and the microbial community composition in a continuous system was evaluated. Two bacterial genera, Clostridium and Streptococcus, were dominant in the microbial community depending on the OLR applied. At low OLR (14.7–44.1 gLactose/L-d), Clostridium sp. was dominant and directed the system towards the acetate-butyrate fermentation pathway, with a maximum H2 yield of 2.14 molH2/molHexose obtained at 29.4 gLactose/L-d. Under such conditions, the volumetric hydrogen production rate (VHPR) was between 3.2 and 11.6 LH2/L-d. In contrast, relatively high OLR (58.8 and 88.2 gLactose/L-d) favored the dominance of Streptococcus sp. as co-dominant microorganism leading to lactate production. Under these conditions, the formate production was also stimulated serving as a strategy to dispose the surplus of reduced molecules (e.g., NADH2+), which theoretically consumed up to 5.72 LH2/L-d. In such scenario, the VHPR was enhanced (13.7–14.5 LH2/L-d) but the H2 yield dropped to a minimum of 0.74 molH2/molHexose at OLR = 58.8 gLactose/L-d. Overall, this research brings clear evidence of the intrinsic occurrence of metabolic pathways detrimental for biohydrogen production, i.e., lactic acid fermentation and formate production, suggesting the use of low OLR as a strategy to control them.

Keywords

Biohydrogen Dark fermentation Lactic acid bacteria (LAB) Hydrogen-producing bacteria (HPB) Microbial community 

Notes

Acknowledgements

Rodolfo Palomo-Briones is thankful for the PhD scholarship provided by the CONACYT. The authors acknowledge the technical assistance of Gaëlle Santa-Catalina, Dulce Partida Gutiérrez, Guillermo Vidriales Escobar, and Juan Pablo Rodas Ortiz. The infrastructure provided by the CONACYT INFR-2014-01-224220 is also acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

The authors confirm that the article does not contain any studies with human participants or animals.

Supplementary material

253_2018_8737_MOESM1_ESM.pdf (702 kb)
ESM 1 (PDF 702kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.División de Ciencias AmbientalesInstituto Potosino de Investigación Científica y TecnológicaSan Luis PotosíMexico
  2. 2.LBE, INRAUniversité MontpellierNarbonneFrance
  3. 3.Departamento de Ingeniería QuímicaCUCEI-Universidad de GuadalajaraGuadalajaraMexico

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