Abstract
Nitrite-dependent anaerobic methane oxidation (n-damo), which is mediated by “Candidatus Methylomirabilis oxyfera-like” bacteria, is unique in linking the carbon and nitrogen cycles. However, the niche and activity of n-damo bacteria in the mangrove ecosystem have not been confirmed. Here, we report the occurrence of the n-damo process in the mangrove wetland of the Zhangjiang Estuary, China. The widespread occurrence of n-damo bacteria in mangrove wetland was confirmed using real-time quantitative polymerase chain reaction (qPCR) assay, which showed that the abundance of Methylomirabilis oxyfera-like bacterial 16S rRNA and pmoA genes ranged from 2.43 × 106 to 2.09 × 107 and 2.07 × 106 to 3.38 × 107copies per gram of dry soil in the examined sediment cores. The highest amount of targeting genes was all detected in the upper layer (0–20 cm). Phylogenetic analyses of n-damo bacterial 16S rRNA and pmoA genes illustrated the depth-specific distribution and high diversity of n-damo bacteria in the mangrove wetland. Stable isotope experiments further confirmed the occurrence of n-damo in the examined mangrove sediments, and the potential n-damo rates ranged from 25.93 to 704.08 nmol CO2 per gram of dry soil per day at different depths of the sediment cores, with the n-damo being more active in the upper layer of the mangrove sediments. These results illustrate the existence of active M. oxyfera-like bacteria and indicate that the n-damo process is a previously overlooked microbial methane sink in the mangrove wetlands.
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We would like to thank Professor John Hodgkiss of the University of Hong Kong for correcting the English in this manuscript.
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This study was funded by the National Natural Science Foundation of China (nos. 41376117 and 41676105).
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YT, MPZ, and TZ designed study; MPZ, LY, LL, and WZ performed the experiments; MPZ, LY, XL, BH, MKZ, QZ, and HX analyzed data; YT and MPZ wrote the paper.
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Zhang, M., Luo, Y., Lin, L. et al. Molecular and stable isotopic evidence for the occurrence of nitrite-dependent anaerobic methane-oxidizing bacteria in the mangrove sediment of Zhangjiang Estuary, China. Appl Microbiol Biotechnol 102, 2441–2454 (2018). https://doi.org/10.1007/s00253-017-8718-2
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DOI: https://doi.org/10.1007/s00253-017-8718-2