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Volatile organic compounds from endophytic fungi as innovative postharvest control of Fusarium oxysporum in cherry tomato fruits

  • Applied microbial and cell physiology
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Abstract

To assess their potential as biopesticides, the effect on the growth of phytopathogen Fusarium oxysporum of six volatile organic compounds from endophytic fungi was studied in vivo and in vitro; compounds were used both as a mixture and individually. In vivo studies were performed inoculating the pathogen into cherry tomatoes, while the in vitro antifungal effect was studied using agar dilution and gas phase methods. Also, the morphology of the hyphae exposed to these compounds was analyzed. Moreover, the possible mechanism of action of these compounds was determined by studying the respiration and cell membrane permeability. Results show that the compounds have a significant concentration-dependent antifungal effect individually and act in a synergic manner. Additionally, changes in cell membrane permeability, damage to the hyphal morphology, and an inhibitory effect on the respiration were observed. The mixture of the six compounds may be used for postharvest control of F. oxysporum in tomatoes.

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Acknowledgements

This work was supported by grants CONACyT 179194 and DGAPA-UNAM IN207117. Yoli Mariana Medina-Romero is a doctoral student from Programa de Doctorado en Ciencias Biomédicas, Universidad Nacional Autónoma de México (UNAM) and received fellowship 239871 from CONACYT. We wish to thank Dra. Bertha Tlapal Bolaños from Instituto de Fitosanidad, Colegio de Postgraduados, Montecillo, Estado de México, for the donation of the plant pathogenic microorganism used in the bioassays and to Dra. Elizabeth K. Galván Miranda from Facultad de Química, UNAM, for language revision.

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  1. Instituto de Química, Departamento de Productos Naturales, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, Delegación Coyoacán, Ciudad de México, 04510, México

    Yoli Mariana Medina-Romero, Gonzalo Roque-Flores & Martha Lydia Macías-Rubalcava

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  1. Yoli Mariana Medina-Romero
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  2. Gonzalo Roque-Flores
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  3. Martha Lydia Macías-Rubalcava
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Correspondence to Martha Lydia Macías-Rubalcava.

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Taken in part from the PhD thesis of Y. M. Medina-Romero

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Medina-Romero, Y.M., Roque-Flores, G. & Macías-Rubalcava, M.L. Volatile organic compounds from endophytic fungi as innovative postharvest control of Fusarium oxysporum in cherry tomato fruits. Appl Microbiol Biotechnol 101, 8209–8222 (2017). https://doi.org/10.1007/s00253-017-8542-8

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