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Lactic acid bacteria isolated from yak milk show probiotic potential

Applied Microbiology and Biotechnology volume 101, pages 7635–7652 (2017)Cite this article

Abstract

Probiotic industries strive for new, efficient and promising probiotic strains that impart a positive impact on consumer health. Challenges are persisting in isolation, screening, and selection of the new indigenous probiotic strains. In the present research, we explored the probiotic potential of 17 lactic acid bacteria isolated from Yak milk in a series of in vitro tests. We also demonstrated their health benefits, i.e., cholesterol degradation, lactose digestion, antimicrobial activity, antioxidant, and anticancer activities. Principal component analysis revealed that more than 50% of the strains fulfilled the examined criteria, e.g., survival in acidic pH, bile concentrations, and adherent property. Approximately all the strains produced antimicrobial substances against the maximum number of tested strains including clinical strains. Most strains degraded cholesterol in comparison to the reference probiotic strain whereas strain Yc showed 1.5 times higher the degradation efficiency of the control strain. Lan4 strain exhibited remarkable anticancer activity and induced the maximum apoptosis (87%) in the Hela cells and was non-toxic to the non-cancerous HEK293 cells. Around ten strains showed positive lactose digestion. Overall, this can be concluded that selected lactic acid bacteria revealed excellent probiotic properties along with desirable health benefits. These strains need to be further investigated in details for their application in the development of novel probiotic preparations for the improvement of public health.

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Acknowledgements

We are highly thankful to the Director CSIR-IMTECH and Director CSIR-CSIO for all the facilities provided to conduct this work. We also thank Mr. Deepak Bhatt for technical assistance in 16S rRNA gene sequencing. MK is thankful to Dr. H.K Sardana and Dr. C Ghanshyam for their support and motivation.

Funding information

The research fellowship of MK is granted by University Grants Commission (UGC) and HS by Indian Council of Medical Research (ICMR), Government of India that are highly acknowledged. We also acknowledge the financial support provided by Dr. Amol P Bhondekar (CSIR-CSIO) for sample procurement. Dr. P Anil Kumar is thankful to the project BSC402 and BSC120 for proving funding and facilities.

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  1. Academy of Scientific and Innovative Research (AcSIR), New Delhi, 110020, India

    Manpreet Kaur, Harjodh Singh, Hemraj Nandanwar, Saumya Ray Chaudhuri, Manoj Raje, Sunita Mishra & Anil Kumar Pinnaka

  2. Council of Scientific and Industrial Research (CSIR)—Central Scientific Instruments Organisation, Chandigarh, 160030, India

    Manpreet Kaur, Harjodh Singh & Sunita Mishra

  3. MTCC-Microbial Type Culture Collection & Gene Bank, CSIR-Institute of Microbial Technology, Chandigarh, 160036, India

    Manpreet Kaur, Harjodh Singh, Manoj Jangra, Lakhwinder Kaur, Pallavi Jaswal, Chetna Dureja, Hemraj Nandanwar, Saumya Ray Chaudhuri, Manoj Raje & Anil Kumar Pinnaka

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  1. Manpreet Kaur
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Correspondence to Anil Kumar Pinnaka.

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The manuscript has CSIR-Institute of Microbial Technology (IMTECH) communication number: 080/2016

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Kaur, M., Singh, H., Jangra, M. et al. Lactic acid bacteria isolated from yak milk show probiotic potential. Appl Microbiol Biotechnol 101, 7635–7652 (2017). https://doi.org/10.1007/s00253-017-8473-4

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Keywords

  • Lactic acid bacteria
  • Probiotics
  • Yak milk
  • Cholesterol degradation