Transcriptome analysis of the thermotolerant yeast Kluyveromyces marxianus CCT 7735 under ethanol stress

Diniz, Raphael Hermano Santos; Villada, Juan C.; Alvim, Mariana Caroline Tocantins; Vidigal, Pedro Marcus Pereira; Vieira, Nívea Moreira; Lamas-Maceiras, Mónica; Cerdán, María Esperanza; González-Siso, María-Isabel; Lahtvee, Petri-Jaan; da Silveira, Wendel Batista

doi:10.1007/s00253-017-8432-0

Genomics, Transcriptomics, Proteomics
Published: 03 August 2017

Transcriptome analysis of the thermotolerant yeast Kluyveromyces marxianus CCT 7735 under ethanol stress

Raphael Hermano Santos Diniz¹,
Juan C. Villada¹,
Mariana Caroline Tocantins Alvim¹,
Pedro Marcus Pereira Vidigal²,
Nívea Moreira Vieira^1,2,
Mónica Lamas-Maceiras³,
María Esperanza Cerdán³,
María-Isabel González-Siso³,
Petri-Jaan Lahtvee⁴ &
Wendel Batista da Silveira¹

Applied Microbiology and Biotechnology volume 101, pages 6969–6980 (2017)Cite this article

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Abstract

The thermotolerant yeast Kluyveromyces marxianus displays a potential to be used for ethanol production from both whey and lignocellulosic biomass at elevated temperatures, which is highly alluring to reduce the cost of the bioprocess. Nevertheless, contrary to Saccharomyces cerevisiae, K. marxianus cannot tolerate high ethanol concentrations. We report the transcriptional profile alterations in K. marxianus under ethanol stress in order to gain insights about mechanisms involved with ethanol response. Time-dependent changes have been characterized under the exposure of 6% ethanol and compared with the unstressed cells prior to the ethanol addition. Our results reveal that the metabolic flow through the central metabolic pathways is impaired under the applied ethanol stress. Consistent with these results, we also observe that genes involved with ribosome biogenesis are downregulated and gene-encoding heat shock proteins are upregulated. Remarkably, the expression of some gene-encoding enzymes related to unsaturated fatty acid and ergosterol biosynthesis decreases upon ethanol exposure, and free fatty acid and ergosterol measurements demonstrate that their content in K. marxianus does not change under this stress. These results are in contrast to the increase previously reported with S. cerevisiae subjected to ethanol stress and suggest that the restructuration of K. marxianus membrane composition differs in the two yeasts which gives important clues to understand the low ethanol tolerance of K. marxianus compared to S. cerevisiae.

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22 February 2021
A Correction to this paper has been published: https://doi.org/10.1007/s00253-021-11185-2

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Acknowledgements

This study was supported by the Brazilian Agencies CNPq (National Science and Technology Development Council), CAPES (Coordination for the Improvement of Higher Education Personnel), and FAPEMIG (Foundation for Research Support of the State of Minas Gerais). The work carried out at Universidade da Coruña was cofunded from Xunta de Galicia (Consolidación D.O.G. 10-10-2012. Contract no. 2012/118 and D.O.G 12-20-2016 Contract no ED431C-2016-012) cofinanced by FEDER. The work performed at University of Tartu was funded by European Research Council (project SynBioTEC) and Estonian Research Council (grant PUT1488). The authors thank the Center for Analysis of Biomolecules of Universidade Federal de Viçosa for the equipment and software used in this study.

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Affiliations

Laboratory of Microbial Physiology, Department of Microbiology, Universidade Federal de Viçosa, Vicosa, MG, Brazil
Raphael Hermano Santos Diniz, Juan C. Villada, Mariana Caroline Tocantins Alvim, Nívea Moreira Vieira & Wendel Batista da Silveira
Center for Analysis of Biomolecules, Center for Biological and Health Sciences, Universidade Federal de Viçosa, Vicosa, MG, Brazil
Pedro Marcus Pereira Vidigal & Nívea Moreira Vieira
Exprela Research Group, Facultade de Ciencias and CICA (Centro de Investigacións Científicas Avanzadas), Universidade da Coruña, A Coruña, Spain
Mónica Lamas-Maceiras, María Esperanza Cerdán & María-Isabel González-Siso
Institute of Technology, University of Tartu, Tartu, Estonia
Petri-Jaan Lahtvee

Authors

Raphael Hermano Santos Diniz
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Juan C. Villada
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Mariana Caroline Tocantins Alvim
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Pedro Marcus Pereira Vidigal
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Nívea Moreira Vieira
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Mónica Lamas-Maceiras
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María Esperanza Cerdán
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María-Isabel González-Siso
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Petri-Jaan Lahtvee
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Wendel Batista da Silveira
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Contributions

RHSD, MLM, and NMV executed the bench procedures. PJL, PMPV, and JCV performed the bioinformatics procedures. JCV, PJL, and WBDS analyzed the data. MIGS and MEC supervised the work at the Spanish laboratory and contributed to data interpretation. JCV, MCTA, PJL, and WBDS wrote the manuscript. WBDS designed and supervised the overall of research project. All authors have read and approved the final version of the manuscript.

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Correspondence to Wendel Batista da Silveira.

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Diniz, R.H.S., Villada, J.C., Alvim, M.C.T. et al. Transcriptome analysis of the thermotolerant yeast Kluyveromyces marxianus CCT 7735 under ethanol stress. Appl Microbiol Biotechnol 101, 6969–6980 (2017). https://doi.org/10.1007/s00253-017-8432-0

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Received: 22 March 2017
Revised: 11 July 2017
Accepted: 19 July 2017
Published: 03 August 2017
Issue Date: September 2017
DOI: https://doi.org/10.1007/s00253-017-8432-0

Keywords

Kluyveromyces marxianus
Transcriptome
Ethanol stress
Membrane