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Applied Microbiology and Biotechnology

, Volume 101, Issue 15, pp 6023–6037 | Cite as

Synergistic hydrolysis of xylan using novel xylanases, β-xylosidases, and an α-l-arabinofuranosidase from Geobacillus thermodenitrificans NG80-2

  • Di HuangEmail author
  • Jia Liu
  • Yanfei Qi
  • Kexin Yang
  • Yingying Xu
  • Lu FengEmail author
Biotechnologically relevant enzymes and proteins

Abstract

Lignocellulosic biomass from various types of wood has become a renewable resource for production of biofuels and biobased chemicals. Because xylan is the major component of wood hemicelluloses, highly efficient enzymes to enhance xylan hydrolysis can improve the use of lignocellulosic biomass. In this study, a xylanolytic gene cluster was identified from the crude oil-degrading thermophilic strain Geobacillus thermodenitrificans NG80-2. The enzymes involved in xylan hydrolysis, which include two xylanases (XynA1, XynA2), three β-xylosidases (XynB1, XynB2, XynB3), and one α-l-arabinofuranosidase (AbfA), have many unique features, such as high pH tolerance, high thermostability, and a broad substrate range. The three β-xylosidases were highly resistant to inhibition by product (xylose) accumulation. Moreover, the combination of xylanase, β-xylosidase, and α-l-arabinofuranosidase exhibited the largest synergistic action on xylan degradation (XynA2, XynB1, and AbfA on oat spelt or beechwood xylan; XynA2, XynB3, and AbfA on birchwood xylan). We have demonstrated that the proposed enzymatic cocktail almost completely converts complex xylan to xylose and arabinofuranose and has great potential for use in the conversion of plant biomass into biofuels and biochemicals.

Keywords

Xylan Xylanase β-Xylosidase α-l-Arabinofuranosidase Synergistic action Geobacillus thermodenitrificans NG80-2 

Notes

Acknowledgements

This research was financially supported by the National Basic Research Program of China (973 Program) (Nos. 2013CB733904, 2012CB721001, and 2012CB721101), the National Natural Science Foundation of China (Nos. ​31270133, 31470194, 31371259, 81471904, 31270003 and 31400081), the Tianjin Research Program of Application Foundation and Advanced Technology (15JCQNJC09700) and the Fundamental Research Funds for the Central Universities (No. 65141028).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals.

Supplementary material

253_2017_8341_MOESM1_ESM.pdf (268 kb)
ESM 1 (PDF 267 kb).

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© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.TEDA Institute of Biological Sciences and Biotechnology, Tianjin Economic-Technological Development Area (TEDA)Nankai UniversityTianjinPeople’s Republic of China
  2. 2.Key Laboratory of Molecular Microbiology and TechnologyMinistry of EducationTianjinPeople’s Republic of China
  3. 3.Tianjin Key Laboratory of Microbial Functional GenomicsTianjinPeople’s Republic of China
  4. 4.SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin)Nankai UniversityTianjinPeople’s Republic of China

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