Applied Microbiology and Biotechnology

, Volume 101, Issue 14, pp 5749–5763

Heavy metal accumulation by Saccharomyces cerevisiae cells armed with metal binding hexapeptides targeted to the inner face of the plasma membrane

  • Lavinia Liliana Ruta
  • Ralph Kissen
  • Ioana Nicolau
  • Aurora Daniela Neagoe
  • Andrei José Petrescu
  • Atle M. Bones
  • Ileana Cornelia Farcasanu
Applied genetics and molecular biotechnology

Abstract

Accumulation of heavy metals without developing toxicity symptoms is a phenotype restricted to a small group of plants called hyperaccumulators, whose metal-related characteristics suggested the high potential in biotechnologies such as bioremediation and bioextraction. In an attempt to extrapolate the heavy metal hyperaccumulating phenotype to yeast, we obtained Saccharomyces cerevisiae cells armed with non-natural metal-binding hexapeptides targeted to the inner face of the plasma membrane, expected to sequester the metal ions once they penetrated the cell. We describe the construction of S. cerevisiae strains overexpressing metal-binding hexapeptides (MeBHxP) fused to the carboxy-terminus of a myristoylated green fluorescent protein (myrGFP). Three non-toxic myrGFP-MeBHxP (myrGFP-H6, myrGFP-C6, and myrGFP-(DE)3) were investigated against an array of heavy metals in terms of their effect on S. cerevisiae growth, heavy metal (hyper) accumulation, and capacity to remove heavy metal from contaminated environments.

Keywords

Heavy metal Metal-binding hexapeptide Accumulation Saccharomyces cerevisiae 

Supplementary material

253_2017_8335_MOESM1_ESM.pdf (407 kb)
ESM 1(PDF 407 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Lavinia Liliana Ruta
    • 1
  • Ralph Kissen
    • 2
  • Ioana Nicolau
    • 1
  • Aurora Daniela Neagoe
    • 3
  • Andrei José Petrescu
    • 4
  • Atle M. Bones
    • 2
  • Ileana Cornelia Farcasanu
    • 1
  1. 1.Faculty of ChemistryUniversity of BucharestBucharestRomania
  2. 2.Cell, Molecular Biology and Genomics Group, Department of BiologyNorwegian University of Science and TechnologyTrondheimNorway
  3. 3.Faculty of BiologyUniversity of BucharestBucharestRomania
  4. 4.Institute of Biochemistry of the Romanian AcademyBucharestRomania

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