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Application of recombinant Pediococcus acidilactici BD16 (fcs +/ech +) for bioconversion of agrowaste to vanillin

Applied Microbiology and Biotechnology volume 101, pages 5615–5626 (2017)Cite this article

Abstract

Biotechnological production of vanillin is gaining momentum as the natural synthesis of vanillin that is very expensive. Ferulic acid (FA), a costly compound, is used as the substrate to produce vanillin biotechnologically and the making process is still expensive. Therefore, this study investigated the practical use of an agrobiomass waste, rice bran, and provides the first evidence of a cost-effective production of vanillin within 24 h of incubation using recombinant Pediococcus acidilactici BD16 (fcs +/ech +). Introduction of two genes encoding feruloyl CoA synthetase and enoyl CoA hydratase into the native strain increased vanillin yield to 4.01 g L−1. Bioconversion was monitored through the transformation of phenolic compounds. A hypothetical metabolic pathway of rice bran during the vanillin bioconversion was proposed with the inserted pathway from ferulic acid to vanillin and compared with that of other metabolic engineered strains. These results could be a gateway of using recombinant lactic acid bacteria for industrial production of vanillin from agricultural waste.

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Acknowledgements

Authors acknowledge University Grants Commission, New Delhi, India for providing UGC major research project to Dr. Baljinder Kaur and meritorious scholarship to Mr. Debkumar Chakraborty and Masafumi Noda for providing cloning vector pLES003.

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Affiliations

  1. Department of Biotechnology, Punjabi University, Patiala, Punjab, India

    Debkumar Chakraborty & Baljinder Kaur

  2. Sino-Forest Applied Research Centre for Pearl River Delta Environment and Department of Biology, Hong Kong Baptist University, Hong Kong SAR, China

    Debkumar Chakraborty, Ammaiyappan Selvam, Jonathan Woon Chung Wong & Obulisamy Parthiba Karthikeyan

  3. Department of Plant Science, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, India

    Ammaiyappan Selvam

Authors
  1. Debkumar Chakraborty
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  2. Ammaiyappan Selvam
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  3. Baljinder Kaur
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  4. Jonathan Woon Chung Wong
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  5. Obulisamy Parthiba Karthikeyan
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Corresponding author

Correspondence to Baljinder Kaur.

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All the authors mutually agree to submit the manuscript to Applied Microbiology and Biotechnology.

Funding

The authors acknowledge the UGC major research project entitled “Metabolic engineering of a lactic acid bacterial isolate for biotransformation of ferulic acid to vanillin” to Dr. Baljinder Kaur (F.39-271/2010(SR)).

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Chakraborty, D., Selvam, A., Kaur, B. et al. Application of recombinant Pediococcus acidilactici BD16 (fcs +/ech +) for bioconversion of agrowaste to vanillin. Appl Microbiol Biotechnol 101, 5615–5626 (2017). https://doi.org/10.1007/s00253-017-8283-8

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  • DOI: https://doi.org/10.1007/s00253-017-8283-8

Keywords

  • Agro waste
  • Rice bran
  • Vanillin
  • Recombinant Pediococcus acidilactici
  • Phenolic metabolites