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Valorization of spent oyster mushroom substrate and laccase recovery through successive solid state cultivation of Pleurotus, Ganoderma, and Lentinula strains

  • Environmental biotechnology
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Abstract

Spent mushroom substrate (SMS) of Pleurotus ostreatus was supplemented with wheat bran and soybean flour in various proportions to obtain C/N ratios of 10, 20, and 30, and their effect was evaluated in successive cultivation of Pleurotus ostreatus, Pleurotus pulmonarius, Ganoderma adspersum, Ganoderma resinaceum, and Lentinula edodes strains with respect to mycelium growth rate, biomass concentration, recovery of the enzyme laccase and crude exopolysaccharides, and also with additional fruiting body production. All fungi showed the highest growth rate on unamended SMS (C/N 30), with G. resinaceum being the fastest colonizer (Kr = 9.84 mm day−1), while biomass concentration maximized at C/N 10. Moreover, supplementation affected positively laccase activity, with P. pulmonarius furnishing the highest value (44,363.22 U g−1) at C/N 20. On the contrary, L. edodes growth, fruiting, and laccase secretion were not favored by SMS supplementation. Fruiting body formation was promoted at C/N 30 for Ganoderma and at C/N 20 for Pleurotus species. Exopolysaccharide production of further studied Pleurotus strains was favored at a C/N 20 ratio, at the initial stage of SMS colonization. The obtained results support the potential effective utilization of supplemented SMS for laccase production from Ganoderma spp. and for new fruiting body production of Pleurotus spp.

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Acknowledgements

This work was supported by the Project “Research & Technology Development Innovation Projects”—AgroETAK, MIS 453350, in the framework of the Operational Program “Human Resources Development.” It was co-funded by the European Social Fund through the National Strategic Reference Framework (Research Funding Program 2007–2013) coordinated by the Hellenic Agricultural Organization—DEMETER (Institute of Technology of Agricultural Products/Laboratory of Edible Fungi).

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  1. Laboratory of Edible Fungi, Institute of Technology of Agricultural Products (ITAP), Hellenic Agricultural Organization—Demeter, 1 Sofokli Venizelou Street, 14123, Lykovryssi, Attiki, Greece

    Christina N. Economou, Panagiota A. Diamantopoulou & Antonios N. Philippoussis

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  1. Christina N. Economou
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  2. Panagiota A. Diamantopoulou
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  3. Antonios N. Philippoussis
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Correspondence to Panagiota A. Diamantopoulou.

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Economou, C.N., Diamantopoulou, P.A. & Philippoussis, A.N. Valorization of spent oyster mushroom substrate and laccase recovery through successive solid state cultivation of Pleurotus, Ganoderma, and Lentinula strains. Appl Microbiol Biotechnol 101, 5213–5222 (2017). https://doi.org/10.1007/s00253-017-8251-3

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