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Genetically engineered Escherichia coli Nissle 1917 synbiotic counters fructose-induced metabolic syndrome and iron deficiency

Applied Microbiology and Biotechnology volume 101, pages 4713–4723 (2017)Cite this article

Abstract

Consumption of fructose leads to metabolic syndrome, but it is also known to increase iron absorption. Present study investigates the effect of genetically modified Escherichia coli Nissle 1917 (EcN) synbiotic along with fructose on non-heme iron absorption. Charles foster rats weighing 150–200 g were fed with iron-deficient diet for 2 months. Probiotic treatment of EcN (pqq) and EcN (pqq-glf-mtlK) was given once per week, 109 cells after 2 months with fructose in drinking water. Iron levels, blood, and liver parameters for oxidative stress, hyperglycemia, and dyslipidemia were estimated. Transferrin-bound iron levels in the blood decreased significantly after 10 weeks of giving iron-deficient diet. Probiotic treatment of EcN (pqq-glf-mtlK) and fructose together led to the restoration of normal transferrin-bound iron levels and blood and hepatic antioxidant levels as compared to iron-deficient control group. The probiotic also led to the restoration of body weight along with levels of serum and hepatic lipid, blood glucose, and antioxidant in the blood and liver as compared to iron-deficient control group. Restoration of liver injury marker enzymes was also seen. Administration of EcN-producing PQQ and mannitol dehydrogenase enzyme together with fructose led to increase in the transferrin-bound iron levels in the blood and amelioration of consequences of metabolic syndrome caused due to fructose consumption.

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Acknowledgement

We are grateful to Dr. Rer. Nat. Ulrich Sonnenborn, Ardeypharm GmbH, Loerfeldstrabe 20, Herdecke (Germany), for providing the Escherichia coli Nissle 1917 and the DBT-ILSPARE program for carrying out real-time PCR.

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Authors and Affiliations

  1. Molecular Microbial Biochemistry Laboratory (M.M.B.L.), Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390002, India

    Archana Somabhai Chaudhari, Ruma Raghuvanshi & G. Naresh Kumar

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  1. Archana Somabhai Chaudhari
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  2. Ruma Raghuvanshi
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  3. G. Naresh Kumar
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Correspondence to G. Naresh Kumar.

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Funding

Archana Somabhai Chaudhari and Ruma Raghuvanshi are supported by Senior Research Fellowship of Department Of Biotechnology (DBT-JRF/2011–12/304) and Indian Council Of Medical Research (3/1/3/JRF-2012/HRD), respectively.

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The authors declare that they have no conflict of interest.

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All applicable institutional guidelines for the care and use of animals were followed.

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Chaudhari, A.S., Raghuvanshi, R. & Kumar, G.N. Genetically engineered Escherichia coli Nissle 1917 synbiotic counters fructose-induced metabolic syndrome and iron deficiency. Appl Microbiol Biotechnol 101, 4713–4723 (2017). https://doi.org/10.1007/s00253-017-8207-7

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  • DOI: https://doi.org/10.1007/s00253-017-8207-7

Keywords

  • E. coli Nissle 1917
  • Iron
  • Fructose
  • SCFA
  • Synbiotic