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Applied Microbiology and Biotechnology

, Volume 101, Issue 10, pp 4151–4161 | Cite as

Characterization and application of endogenous phase-dependent promoters in Bacillus subtilis

  • Sen Yang
  • Guocheng Du
  • Jian Chen
  • Zhen Kang
Applied genetics and molecular biotechnology

Abstract

Bacillus subtilis as an important host has been widely used in synthetic biology, metabolic engineering, and production of industrial enzymes. To fully take advantage of this organism, 114 endogenous putative promoters were measured with a green fluorescent protein reporter and four classes of phase-dependent promoters (class I: exponential phase; class II: middle-log and early stationary phases; class III: lag-log and stationary phases; class IV: stationary phase) with different strengths were identified. The transcriptional strengths ranged from 0.03 to 2.03-fold of that of the commonly used strong promoter P43. On this basis, the temperature (for instance P bltD , P ydaD , and P gerBC ) and pH (such as P abrB , P ydjO , and P opuE ) inducible phase-dependent promoters were further identified and characterized. In comparison, P abrB (class I), P spoVG (class II), and P lytR (class III) achieved the highest expression levels of esterase, keratinase, and alkaline polygalacturonate lyase, respectively. The constructed phase-dependent promoter library should have great application potentials for enzyme production, metabolic engineering, and synthetic biology.

Keywords

Bacillus subtilis Microbial cell factory Phase-dependent promoter Synthetic biology 

Notes

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (31670092), the Natural Science Foundation of Jiangsu Province (BK20141107), a grant from the Key Technologies R&D Program of Jiangsu Province, China, (BE2014607), and Program for Changjiang Scholars and Innovative Research Team in University (No. IRT_15R26).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2017_8142_MOESM1_ESM.pdf (231 kb)
ESM 1 (PDF 230 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiChina
  2. 2.Synergetic Innovation Center of Food Safety and NutritionJiangnan UniversityWuxiChina

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