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Applied Microbiology and Biotechnology

, Volume 101, Issue 6, pp 2533–2547 | Cite as

Development and validation of a new dynamic in vitro model of the piglet colon (PigutIVM): application to the study of probiotics

  • Mickaël Alain Fleury
  • Olivier Le Goff
  • Sylvain Denis
  • Frédérique Chaucheyras-Durand
  • Eric Jouy
  • Isabelle Kempf
  • Monique Alric
  • Stéphanie Blanquet-DiotEmail author
Methods and protocols

Abstract

For ethical, technical, regulatory, and cost reasons, in vitro methods are increasingly used as an alternative to in vivo experimentations. The aim of the present study was to validate, according to in vivo data in living animals, a new in vitro model of the piglet colon, the PigutIVM, under both control conditions and antibiotic disturbance by the widely used colistin. The PigutIVM reproduces the main biotic and abiotic parameters of the piglet colon: temperature, pH, retention time, supply of ileal effluents, complex, and metabolically active microbiota and self-maintained anaerobiosis. Under both control and antibiotic-treated conditions, qPCR analyses showed that the main bacterial populations of piglet gut microbiota were similar in vitro and in vivo, with Pearson correlation coefficient higher than 0.9. During colistin administration, both in piglets and in the in vitro model, a significant decrease in Escherichia coli populations was observed together with changes in microbial composition of subdominant populations. SCFA concentrations were similar in vitro and in vivo and were not modified by colistin. Interestingly, the administration of the probiotic Saccharomyces cerevisiae var. boulardii CNCM I-1079 led in vitro to a decrease in E. coli levels, as previously observed when the antibiotic treatment was applied. This new in vitro model of the piglet colon provides a flexible, reproducible, and cost-effective tool for the screening of drugs or new dietary compounds, such as pre- or probiotics. It will be helpful for researchers, feed producers, or veterinarians when developing innovative non-antibiotic strategies.

Keywords

In vitro model Piglet Gut microbiota Colistin Probiotic Saccharomyces cerevisiae var. boulardii 

Notes

Acknowledgments

This study was supported by the Côtes d’Armor General Council and the Brittany and Auvergne regions.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving animals

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Supplementary material

253_2017_8122_MOESM1_ESM.pdf (382 kb)
ESM 1 (PDF 382 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Mickaël Alain Fleury
    • 1
    • 2
    • 3
  • Olivier Le Goff
    • 1
  • Sylvain Denis
    • 1
  • Frédérique Chaucheyras-Durand
    • 1
    • 4
  • Eric Jouy
    • 2
    • 3
  • Isabelle Kempf
    • 2
    • 3
  • Monique Alric
    • 1
  • Stéphanie Blanquet-Diot
    • 1
    Email author
  1. 1.UMR UCA INRA 454 MEDISCentre de Recherche en Nutrition Humaine, Université Clermont AuvergneClermont-FerrandFrance
  2. 2.Laboratoire de Ploufragan-Plouzané, ANSESPloufraganFrance
  3. 3.Université Bretagne LoireRennesFrance
  4. 4.Lallemand Animal NutritionBlagnacFrance

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