Applied Microbiology and Biotechnology

, Volume 101, Issue 7, pp 2893–2903 | Cite as

Xyloglucan breakdown by endo-xyloglucanase family 74 from Aspergillus fumigatus

  • André Ricardo de Lima Damasio
  • Marcelo Ventura Rubio
  • Thiago Augusto Gonçalves
  • Gabriela Felix Persinoti
  • Fernando Segato
  • Rolf Alexander Prade
  • Fabiano Jares Contesini
  • Amanda Pereira de Souza
  • Marcos Silveira Buckeridge
  • Fabio Marcio Squina
Biotechnologically relevant enzymes and proteins

Abstract

Xyloglucan is the most abundant hemicellulose in primary walls of spermatophytes except for grasses. Xyloglucan-degrading enzymes are important in lignocellulosic biomass hydrolysis because they remove xyloglucan, which is abundant in monocot-derived biomass. Fungal genomes encode numerous xyloglucanase genes, belonging to at least six glycoside hydrolase (GH) families. GH74 endo-xyloglucanases cleave xyloglucan backbones with unsubstituted glucose at the −1 subsite or prefer xylosyl-substituted residues in the −1 subsite. In this work, 137 GH74-related genes were detected by examining 293 Eurotiomycete genomes and Ascomycete fungi contained one or no GH74 xyloglucanase gene per genome. Another interesting feature is that the triad of tryptophan residues along the catalytic cleft was found to be widely conserved among Ascomycetes. The GH74 from Aspergillus fumigatus (AfXEG74) was chosen as an example to conduct comprehensive biochemical studies to determine the catalytic mechanism. AfXEG74 has no CBM and cleaves the xyloglucan backbone between the unsubstituted glucose and xylose-substituted glucose at specific positions, along the XX motif when linked to regions deprived of galactosyl branches. It resembles an endo-processive activity, which after initial random hydrolysis releases xyloglucan-oligosaccharides as major reaction products. This work provides insights on phylogenetic diversity and catalytic mechanism of GH74 xyloglucanases from Ascomycete fungi.

Keywords

Fungal glucanases Xyloglucanases Xyloglucan specific Aspergillus Endo-processive 

Supplementary material

253_2016_8014_MOESM1_ESM.pdf (407 kb)
ESM 1(PDF 406 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • André Ricardo de Lima Damasio
    • 1
    • 2
  • Marcelo Ventura Rubio
    • 1
    • 2
  • Thiago Augusto Gonçalves
    • 1
    • 2
  • Gabriela Felix Persinoti
    • 1
  • Fernando Segato
    • 1
    • 3
  • Rolf Alexander Prade
    • 4
  • Fabiano Jares Contesini
    • 1
  • Amanda Pereira de Souza
    • 5
  • Marcos Silveira Buckeridge
    • 5
  • Fabio Marcio Squina
    • 1
  1. 1.Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM)CampinasBrazil
  2. 2.Department of Biochemistry and Tissue Biology, Institute of BiologyState University of CampinasCampinasBrazil
  3. 3.Departamento de Biotecnologia, Escola de Engenharia de Lorena (EEL)Universidade de São Paulo (USP)LorenaBrazil
  4. 4.Department of Microbiology and Molecular GeneticsOklahoma State UniversityStillwaterUSA
  5. 5.Laboratório de Fisiologia e Ecologia de Plantas (LAFIECO), Departamento de Botânica, Instituto de BiociênciasUniversidade de São Paulo (USP)São PauloBrazil

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