Applied Microbiology and Biotechnology

, Volume 101, Issue 4, pp 1477–1485 | Cite as

Development of engineered yeast for biosorption of beer haze-active polyphenols

  • Rudolf Cejnar
  • Kateřina Hložková
  • Lukáš Jelínek
  • Pavel Kotrba
  • Pavel Dostálek
Biotechnologically relevant enzymes and proteins
  • 293 Downloads

Abstract

Compared to most other alcoholic beverages, the shelf life of beer is much more limited due to its instability in the bottle. That instability is most likely to appear as turbidity (haze), even sedimentation, during storage. The haze in beer is mostly caused by colloidal particles formed by interactions between proteins and polyphenols within the beer. Therefore, beers are usually stabilized by removing at least one of these components. We developed and constructed a Saccharomyces cerevisiae strain with a proline-rich QPF peptide attached to the cell wall, using the C-terminal anchoring domain of α-agglutinin. The QPF peptide served to bind polyphenols during fermentation and, thus, to decrease their concentration. Strains displaying QPF were able to bind about twice as much catechin and epicatechin as a control strain displaying only the anchoring domain. All these experiments were done with model solutions. Depending on the concentration of yeast, uptake of polyphenols was 1.7–2.5 times higher. Similarly, the uptake of proanthocyanidins was increased by about 20 %. Since the modification of yeasts with QPF did not affect their fermentation performance under laboratory conditions, the display of QPF appears to be an approach to increase the stability of beer.

Keywords

Saccharomyces cerevisiae QPF peptide Procyanidin removal Beer colloidal stability 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Department of Biotechnology, Faculty of Food and Biochemical TechnologyUniversity of Chemistry and TechnologyPrague 6Czech Republic
  2. 2.Department of Biochemistry and Microbiology, Faculty of Food and Biochemical TechnologyUniversity of Chemistry and TechnologyPrague 6Czech Republic

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