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Overexpression of PMA1 enhances tolerance to various types of stress and constitutively activates the SAPK pathways in Saccharomyces cerevisiae

  • Applied genetics and molecular biotechnology
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Abstract

PMA1 encodes a transmembrane polypeptide that functions to pump protons out of the cell. Ectopic PMA1 overexpression in Saccharomyces cerevisiae enhances tolerance to weak acids, reactive oxygen species (ROS) and ethanol, and changes the following physiological properties: better proton efflux, lower membrane permeability, and lessened internal hydrogen peroxide production. The enhanced stress tolerance was dependent on the mitogen-activated protein kinase (MAPK) Hog1 of the high osmolarity glycerol (HOG) pathway, but not the MAPK Slt2 of the cell wall integrity (CWI) pathway; however, a PMA1 overexpression constitutively activated both Hog1 and Slt2. The constitutive Hog1 activation required the MAPK kinase kinase (MAP3K) Ssk2 of the HOG pathway, but not Ste11 and Ssk22, two other MAP3Ks of the same pathway. The constitutive Slt2 activation did not require Rom2 and the membrane sensors of the CWI pathway, whereas Bck1 was indispensable. The PMA1 overexpression activated the stress response element but not the cyclic AMP response element and the Rlm1 transcription factor. PMA1 overexpression may facilitate the construction of industrial strains with simultaneous tolerance to weak acids, ROS, and ethanol.

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Acknowledgments

We are grateful to Dr. H. Saito (Tokyo University, Tokyo, Japan) for the p8xCRE-LacZ plasmid; Dr. D. Levin (Johns Hopkins University, Baltimore, MD, USA) for the p1434 (2X-RLM1); and Dr. C. Slayman (Yale University, New Haven, CT, USA) for the yeast strain BMY40. This study was partly supported by the BL21 Plus Program funded by the Ministry of Education and National Research Foundation (NRF) of Korea (Creative Academy of Ecoscience, 31Z20130012990), partly by the NRF of Korea grant funded by the Ministry of Science, ICT and Future Planning, Korea (No. NRF-2016R1A2B4008050 ), and partly by the Marine Biomaterials Research Center Grant from the Marine Biotechnology Program funded by the Ministry of Oceans and Fisheries, Korea (No. D11013214H480000100).

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Authors and Affiliations

  1. Interdisciplinary Program of EcoCreative, College of Natural Sciences, Ewha Womans University, Seoul, 03766, South Korea

    Yeji Lee, Olviyani Nasution & Wonja Choi

  2. Department of Life Sciences College of Natural Sciences, Ewha Womans University, Seoul, 03766, South Korea

    Young Mi Lee & Wonja Choi

  3. Department of Pharmacology, School of Medicine, Ajou University, Suwon, 16499, South Korea

    Eunjung Kim & Wankee Kim

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  1. Yeji Lee
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  2. Olviyani Nasution
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Correspondence to Wonja Choi or Wankee Kim.

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Lee, Y., Nasution, O., Lee, Y.M. et al. Overexpression of PMA1 enhances tolerance to various types of stress and constitutively activates the SAPK pathways in Saccharomyces cerevisiae . Appl Microbiol Biotechnol 101, 229–239 (2017). https://doi.org/10.1007/s00253-016-7898-5

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