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Evaluation of technological properties of Enterococcus faecium CECT 8849, a strain isolated from human milk, for the dairy industry

  • Applied microbial and cell physiology
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Abstract

In this work, a variety of biochemical properties of Enterococcus faecium CECT 8849, which had been isolated from breast milk, were analyzed. Its acidifying capacity and proteolytic activity were low but, in contrast, remarkable peptidase and esterase activities were observed. Ethanol and 3-hydroxy-2-butanone were the most abundant volatile compounds found in experimental model cheese manufactured with E. faecium CECT 8849. This strain inhibited the growth of several Listeria monocytogenes and Listeria innocua strains in vitro. Enterocin A and B structural genes were detected in E. faecium CECT 8849. Model fermented milk and cheeses were manufactured from milk inoculated or not with L. innocua CECT 8848 (2.5–3 log10 colony forming units mL−1) using E. faecium CECT 8849 or Lactococcus lactis ESI 153 as starter cultures. Although E. faecium CECT 8849 controlled Listeria growth in both dairy models, it led to lower reduction in Listeria counts when compared with L. lactis ESI 153.

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Authors and Affiliations

  1. Probisearch, 28760 Tres Cantos, Madrid, Spain

    Nivia Cárdenas, Rebeca Arroyo, Juan Miguel Rodríguez & Leonides Fernández

  2. Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040, Madrid, Spain

    Rebeca Arroyo, Juan Miguel Rodríguez & Leonides Fernández

  3. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, INIA, Carretera de La Coruña, km. 7.5, 28040, Madrid, Spain

    Javier Calzada, Ángela Peirotén & Margarita Medina

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  1. Nivia Cárdenas
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  2. Rebeca Arroyo
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  3. Javier Calzada
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  4. Ángela Peirotén
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  5. Margarita Medina
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  6. Juan Miguel Rodríguez
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  7. Leonides Fernández
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Correspondence to Leonides Fernández.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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This study was funded by the FUN-C-FOOD (Consolider-Ingenio 2010) and AGL2007-62042 projects from the Ministerio de Educación y Ciencia (Spain).

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The authors declare that they have no competing interests.

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Cárdenas, N., Arroyo, R., Calzada, J. et al. Evaluation of technological properties of Enterococcus faecium CECT 8849, a strain isolated from human milk, for the dairy industry. Appl Microbiol Biotechnol 100, 7665–7677 (2016). https://doi.org/10.1007/s00253-016-7616-3

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  • DOI: https://doi.org/10.1007/s00253-016-7616-3

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