Applied Microbiology and Biotechnology

, Volume 100, Issue 18, pp 8043–8051 | Cite as

Improvement of thermostability and activity of Trichoderma reesei endo-xylanase Xyn III on insoluble substrates

  • Tomohiko Matsuzawa
  • Satoshi Kaneko
  • Katsuro YaoiEmail author
Biotechnologically relevant enzymes and proteins


Trichoderma reesei Xyn III, an endo-β-1,4-xylanase belonging to glycoside hydrolase family 10 (GH10), is vital for the saccharification of xylans in plant biomass. However, its enzymatic thermostability and hydrolytic activity on insoluble substrates are low. To overcome these difficulties, the thermostability of Xyn III was improved using random mutagenesis and directed evolution, and its hydrolytic activity on insoluble substrates was improved by creating a chimeric protein. In the screening of thermostable Xyn III mutants from a random mutagenesis library, we identified two amino acid residues, Gln286 and Asn340, which are important for the thermostability of Xyn III. The Xyn III Gln286Ala/Asn340Tyr mutant showed xylanase activity even after heat treatment at 60 °C for 30 min or 50 °C for 96 h, indicating a dramatic enhancement in thermostability. In addition, we found that the addition of a xylan-binding domain (XBD) to the C-terminal of Xyn III improved its hydrolytic activity on insoluble xylan.


Xylanase Trichoderma reesei Biomass Xylan Thermostability Xylan-binding domain 



We thank Yuko Chujo and Akiko Yamanaka (AIST) for assistance in screening of thermostable Xyn III mutants and protein purification. This work was supported in part by grants from the New Energy and Industrial Technology Development Organization (NEDO).

Compliance with ethical standards

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

253_2016_7563_MOESM1_ESM.pdf (99 kb)
ESM 1 (PDF 98 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Tomohiko Matsuzawa
    • 1
  • Satoshi Kaneko
    • 2
  • Katsuro Yaoi
    • 1
    Email author
  1. 1.Bioproduction Research InstituteNational Institute of Advanced Industrial Science and Technology (AIST)IbarakiJapan
  2. 2.Department of Subtropical Biochemistry and Biotechnology, Faculty of AgricultureUniversity of the RyukyusOkinawaJapan

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