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Applied Microbiology and Biotechnology

, Volume 100, Issue 13, pp 5829–5838 | Cite as

Extensive hydrolysis of phosphonates as unexpected behaviour of the known His6-organophosphorus hydrolase

  • Ilya V. Lyagin
  • Mariia S. Andrianova
  • Elena N. Efremenko
Biotechnologically relevant enzymes and proteins

Abstract

The catalytic activity of hexahistidine-tagged organophosphorus hydrolase (His6-OPH) in hydrolytic reactions of methylphosphonic acid (MPA) and its monoesters and diesters being decomposition products of R-VX was demonstrated for the first time. The catalytic constants of enzyme in such reactions were determined. The mechanism of C–P bond cleavage in the MPA by His6-OPH was proposed. Such reaction was estimated to be carried out with the soluble and nanocapsulated forms of His6-OPH. His6-OPH was demonstrated to be capable of degrading the key organophosphorus components of reaction masses (RMs) that are produced by the chemical detoxification of R-VX and RMs are multi-substrate mixtures for this enzyme. The kinetic model describing the behaviour of His6-OPH in RMs was proposed and was shown to adequately fit experimental points during degradation of the real samples of RMs.

Keywords

Organophosphorus compound Phosphonate Mechanism Enzymatic degradation Hexahistidine-tagged organophosphorus hydrolase 

Notes

Acknowledgments

This work was financially supported by the Ministry of Education and Science of RF (contract no. 02.515.11.5002). Enzyme-polyelectrolyte complexes were obtained with financial support by the Russian Foundation for Basic Research (grant no. 15-54-54011). Authors extend special thanks to research fellows of 27 Research Center of MD RF (Moscow, Russia) and State Research Institute of Organic Chemistry and Technology (Moscow, Russia) for supporting the work as analytic maintenance and supplying with substrates. Also, authors are grateful to Viktor I. Kholstov (Department of Realization of Conventional Obligation, Ministry of Industry and Trade of RF, Moscow, Russia) for supporting the work in toto.

Compliance with ethical standards

Funding

This work was financially supported by the Ministry of Education and Science of RF (contract no. 02.515.11.5002) and by the Russian Foundation for Basic Research (grant no. 15–54-54,011).

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animal rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2016_7407_MOESM1_ESM.pdf (200 kb)
ESM 1 (PDF 199 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Chemistry DepartmentLomonosov Moscow State UniversityMoscowRussia
  2. 2.Institute of Biochemical Physics RASMoscowRussia
  3. 3.SMC Technological Center MIETZelenogradRussia

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