Applied Microbiology and Biotechnology

, Volume 100, Issue 15, pp 6631–6642 | Cite as

Multiple effects of trace elements on methanogenesis in a two-phase anaerobic membrane bioreactor treating starch wastewater

  • Dawei Yu
  • Chao Li
  • Lina Wang
  • Junya Zhang
  • Jing Liu
  • Yuansong Wei
Biotechnological products and process engineering


For enhancing anaerobic membrane bioreactor (AnMBR) treating food processing wastewater due to speed-limited methanogenesis step, multiple effects of trace element (TE) supplementation on methanogenesis of a two-phase AnMBR were firstly investigated in batch tests. TE supplementation included individual element, combination and recovery of Fe, Ni, Co, Cu and Zn supplementation. Multiple effects of TE supplementation were highest stimulated by 22.4 ± 5.6 % (TE313) for chemical oxygen demand (COD) removal, 43.1 ± 12.5 % (TE303) for specific methanogenic activity (SMA) and 13.9 ± 3.7 % (TE405) for biomass growth, respectively, although only 7.5 ± 0.6 % (TE106) for methane production. Dosage of TEs played a critical role in methane production, COD removal and biomass growth of the AnMBR’s methanogenesis. Low dosages of TE supplementation improved the COD removal and slightly stimulated the COD bioconverting to methane and biomass, but their specific methanation activities were inhibited in the initial rapid methanogenesis stage. Several methanation functional species were increased in abundance like Methanosarcina and Methanoculleus.


Methanogenesis Trace element Biochemical methanation potential High-throughput sequencing 



This work was funded by Major Science and Technology Program for Water Pollution Control and Treatment of China (2012ZX07203-002; 2015ZX07203-005) and China-EU Innovation Funding for Small and Medium Size Enterprise (SQ2013ZOA000002). The authors also would like to thank Mingxing Zhao for English proofreading.

Compliance with ethical standards

Human and animal rights and informed consent

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare that there are no conflicts of interest.

Supplementary material

253_2016_7289_MOESM1_ESM.pdf (834 kb)
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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijingChina
  2. 2.Department of BiotechnologyLund UniversityLundSweden
  3. 3.School of EnvironmentBeijing Normal UniversityBeijingChina
  4. 4.Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijingChina

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