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L-valine, an antialgal amino acid from Streptomyces jiujiangensis JXJ 0074T

  • Environmental biotechnology
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Abstract

An antialgal compound was isolated from the cultured broth of Streptomyces jiujiangensis JXJ 0074T by using bioassay methods. Based on the data of 1H-NMR, 13C-NMR, ESI-MS, and thin layer chromatography, the active compound was identified as L-valine, which showed antialgal activity mainly against Microcystis. L-valine exhibited greater antialgal activities than both L-lysine and copper sulfate (CuSO4) did on Microcystis aeruginosa lawn. However, M. aeruginosa recovered growth earlier with higher growth rate in L-valine treatment than in L-lysine treatment. L-valine dissipated completely within 2 days, much quicker than L-lysine (6 days), which resulted in the lysing of more than 80 % M. aeruginosa cells and the release of amount of intracellular microcystin-LR (MC-LR) within 2 days. As a resultant, the extracellular MC-LR content was more than twice of the control from day 1 to 5. Exposure to L-valine significantly promoted the synthesis of MC-LR. L-lysine also promoted the release and synthesis of MC-LR with much lesser efficiency than L-valine. L-valine could damage Microcystis severely, causing perforation and collapse of M. aeruginosa cells and decrease of the chlorophyll. The superoxide dismutase (SOD) activity in L-valine-treated cells of M. aeruginosa initially increased with 32.94 ± 3.37 % higher than the control after 36 h and then decreased quickly. However, the increase rate of superoxide anion radical (O2 −) was much higher than that of SOD, which resulted in serious lipid peroxidation and accumulation of malondialdehyde (MDA). To our knowledge, this is the first report showing L-valine active against cyanobacteria.

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Acknowledgments

This research was supported by the Natural Science Foundation of China (No. 31060010), projects of China tobacco Yunnan industrial Co. Ltd. (2014YL01 and 2015CP01), the Deanship of Scientific Research at King Saud University through the research group no. RGP-205, Programs of the Education Department (No. GJJ10619) and the Science and Technology Department (No. 20111BBG70012-4) and the Environmental Conservation Department (NO. JXHBKJ2013-14) of Jiangxi Province of China, and project of Jiujiang University (No. 201511). W-J Li was also supported by project supported by Guangdong Province Higher Vocational Colleges and Schools Pearl River Scholar Funded Scheme (2014).

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    1. College of Life Science, Jiujiang University, Jiujiang, 332000, People’s Republic of China

      Bing-Huo Zhang, Han-Quan Li, Jian-Yuan Yang & Dai-Ming Zha

    2. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

      Bing-Huo Zhang, Min Xiao & Wen-Jun Li

    3. China Tobacco Yunnan Industrial Co. Ltd, Kunming, 650231, People’s Republic of China

      Wei Chen, Yan-Qing Duan & Rui Gao

    4. Bioproducts Research Chair (BRC), College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

      Wael N.Hozzein

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    It is the original work of the authors. The work described has not been submitted elsewhere for publication, in whole or in part, and all authors listed carry out the data analysis and manuscript writing and “This article does not contain any studies with human participants or animals performed by any of the authors”. Moreover, all authors read and approved the final manuscript. The authors declare that they have no direct or indirect conflicts of interest.

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    Zhang, BH., Chen, W., Li, HQ. et al. L-valine, an antialgal amino acid from Streptomyces jiujiangensis JXJ 0074T . Appl Microbiol Biotechnol 100, 4627–4636 (2016). https://doi.org/10.1007/s00253-015-7150-8

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