High zinc ion supplementation of more than 30 μM can increase monoclonal antibody production in recombinant Chinese hamster ovary DG44 cell culture
Effects of high ZnSO4·7H2O supplementation on cell growth and monoclonal antibody (mAb) production in chemically defined suspension cultures of recombinant Chinese hamster ovary (rCHO) DG44 cells were examined. The supplementation of ZnSO4·7H2O up to 120 μM gradually increased specific mAb production rate of rCHO DG44 cells in the early growth phase (0–4 days of culture). The ZnSO4·7H2O concentration for enhancing mAb production without any cytotoxic effects on cell growth was 30–60 μM. In addition of 60 μM ZnSO4·7H2O to in-house protein-free medium and in-house chemically defined medium, mAb production was increased 2.0-fold and 6.5-fold, respectively. Moreover, addition of ZnSO4·7H2O to three kinds of commercial chemically defined media yielded a greater than 1.2-fold enhancement of mAb production. These data indicate that simple supplementation of a relatively high zinc ion concentration to cell culture media without significant changes of rCHO DG44 cell culture process can be useful for achieving high production of mAb.
KeywordsChemically defined medium Chinese hamster ovary cells Monoclonal antibody production Suspension culture Zinc ion
The authors thank Green Cross Corp. (Kyonggi, Korea) for providing rCHO cell lines.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Lee GM, Hwang SO (2004) Heterologous expression of genes in Chinese hamster ovary cells. In: Baneyx F (ed) Protein expression technologies: current status and future trends. Horizon Bioscience, Norfolk, pp. 427–460Google Scholar
- Van DyK DD, Misztal DR, Wilkins MR, Mackintosh JA, Poljak A, Varnai JC, Teber E, Walsh BJ, Gray PP (2003) Identification of cellular changes associated with increased production of human growth hormone in a recombinant Chinese hamster ovary cell line. Proteomics 3:147–156CrossRefPubMedGoogle Scholar
- Zuqueli R, Prieto C, Etcheverrigaray M, Kratje R (2006) Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN-β production by mammalian cell culture. Lat Am Appl Res 36:321–327Google Scholar