Applied Microbiology and Biotechnology

, Volume 100, Issue 3, pp 1469–1478 | Cite as

Microbial community dynamics in Baolige oilfield during MEOR treatment, revealed by Illumina MiSeq sequencing

  • Jing You
  • Gang Wu
  • Fuping Ren
  • Qi Chang
  • Bo YuEmail author
  • Yanfen Xue
  • Bozhong MuEmail author
Environmental biotechnology


This study was carried out to understand microbial diversity and function in the microbial enhanced oil recovery (MEOR) process and to assess the impact of MEOR treatment on the microbial community in an oil reservoir. The Illumina MiSeq-based method was used to investigate the structure and dynamics of the microbial community in a MEOR-treated block of the Baolige oilfield, China. The results showed that microbial diversity was high and that 23 phyla occurred in the analyzed samples. Proteobacteria, Firmicutes, Bacteroidetes, Thermotogae, and Euryarchaeota were present in relatively high abundance in all analyzed samples. Injection of bacteria and nutrients resulted in interesting changes in the composition of the microbial community. During MEOR treatment, the community was dominated by the known hydrocarbon-utilizing genera Pseudomonas and Acinetobacter. After the treatment, the two genera decreased in abundance over time while Methanobacteriaceae, as well as known syntrophic genera such as Syntrophomonas, Pelotomaculum, Desulfotomaculum, and Thermacetogenium gradually increased. The change in dominant microbial populations indicated the presence of a succession of microbial communities over time, and the hydrocarbon degradation and syntrophic oxidation of acetate and propionate to methane in the MEOR-treated oilfield. This work contributes to a better understanding of microbial processes in oil reservoirs and helps to optimize MEOR technology.


MEOR-treated oil reservoir High-throughput sequencing Microbial community Microbial community dynamics 



This work was funded by grants from Huabei Oilfield Ltd. (Grant No. 2014E-3507) and the National Natural Science Foundation of China (31170116).

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors do not have potential conflict of interest to disclose.

Supplementary material

253_2015_7073_MOESM1_ESM.pdf (477 kb)
ESM 1 (PDF 476 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Petroleum Production Engineering Institute of Huabei Oilfield LtdRenqiuChina
  2. 2.Institute of MicrobiologyChinese Academy of SciencesBeijingChina
  3. 3.School of Chemistry and Molecular EngineeringEast China University of Science and TechnologyShanghaiChina

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