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Dye-linked D-amino acid dehydrogenases: biochemical characteristics and applications in biotechnology

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Abstract

Dye-linked D-amino acid dehydrogenases (Dye-DADHs) catalyze the dehydrogenation of free D-amino acids in the presence of an artificial electron acceptor. Although Dye-DADHs functioning in catabolism of L-alanine and as primary enzymes in electron transport chains are widely distributed in mesophilic Gram-negative bacteria, biochemical and biotechnological information on these enzymes remains scanty. This is in large part due to their instability after isolation. On the other hand, in the last decade, several novel types of Dye-DADH have been found in thermophilic bacteria and hyperthermophilic archaea, where they contribute not only to L-alanine catabolism but also to the catabolism of other amino acids, including D-arginine and L-hydroxyproline. In this minireview, we summarize recent developments in our understanding of the biochemical characteristics of Dye-DADHs and their specific application to electrochemical biosensors.

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Acknowledgments

We thank Profs. Tomoki Yabutani and Junko Motonaka for their crucial contributions to the application of Dye-DADH.

Compliance with ethical standards

This work was supported by JSPS KAKENHI Grant Number 25870271.

Conflict of interest

The authors declare that they have no competing interests.

Human and animal rights and informed consent

This article does not contain any studies with human participants or animals performed by any of the authors.

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Authors and Affiliations

  1. Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui, 910-8507, Japan

    Takenori Satomura & Shin-ichiro Suye

  2. Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan

    Haruhiko Sakuraba

  3. Department of Frontier Fiber Technology and Sciences, Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui, 910-8507, Japan

    Shin-ichiro Suye

  4. Department of Biomedical engineering, Faculty of Engineering, Osaka Institute of Technology, Ohmiya, 5-16-1 Asahi-ku, Ohsaka, 535-8585, Japan

    Toshihisa Ohshima

Authors
  1. Takenori Satomura
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  2. Haruhiko Sakuraba
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  3. Shin-ichiro Suye
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  4. Toshihisa Ohshima
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Correspondence to Takenori Satomura.

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Satomura, T., Sakuraba, H., Suye, Si. et al. Dye-linked D-amino acid dehydrogenases: biochemical characteristics and applications in biotechnology. Appl Microbiol Biotechnol 99, 9337–9347 (2015). https://doi.org/10.1007/s00253-015-6944-z

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  • DOI: https://doi.org/10.1007/s00253-015-6944-z

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