Evaluation of soil bioremediation techniques in an aged diesel spill at the Antarctic Peninsula
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Many areas on the Antarctic continent already suffer from the direct and indirect influences of human activities. The main cause of contamination is petroleum hydrocarbons because this compound is used as a source of energy at the many research stations around the continent. Thus, the current study aims to evaluate treatments for bioremediation (biostimulation, bioaugmentation, and bioaugmentation + biostimulation) using soils from around the Brazilian Antarctic Station “Comandante Ferraz” (EACF), King George Island, Antarctic Peninsula. The experiment lasted for 45 days, and at the end of this period, chemical and molecular analyses were performed. Those analyses included the quantification of carbon and nitrogen, denaturing gradient gel electrophoresis (DGGE) analysis (with gradient denaturation), real-time PCR, and quantification of total hydrocarbons and polyaromatics. Molecular tests evaluated changes in the profile and quantity of the rrs genes of archaea and bacteria and also the alkB gene. The influence of the treatments tested was directly related to the type of soil used. The work confirmed that despite the extreme conditions found in Antarctic soils, the bacterial strains degraded hydrocarbons and bioremediation treatments directly influenced the microbial communities present in these soils even in short periods. Although the majority of the previous studies demonstrate that the addition of fertilizer seems to be most effective at promoting bioremediation, our results show that for some conditions, autochthonous bioaugmentation (ABA) treatment is indicated. This work highlights the importance of understanding the processes of recovery of contaminated environments in polar regions because time is crucial to the soil recovery and to choosing the appropriate treatment.
KeywordsSoil Bioremediation Antarctica Diesel
This work integrates the National Institute of Science and Technology Antarctic Environmental Research (INCT-APA) that receives scientific and financial support from the National Council for Research and Development (CNPq process: no. 574018/2008-5) and Carlos Chagas Research Support Foundation of the State of Rio de Janeiro (FAPERJ). The authors also acknowledge the support of the Brazilian Ministries of Science, Technology and Innovation (MCTI), of Environment (MMA) and Inter-Ministry Commission for Sea Resources (CIRM).
Conflict of interest
The authors declare that they have no conflict of interest.
- Chénier MR, Beaumier D, Roy R, Driscoll BT, Lawrence JR, Greer CW (2003) Impact of seasonal variations and nutrient inputs on nitrogen cycling and degradation of hexadecane by replicated river biofilms. Appl Environ Microbiol 69:5170–5177. doi: 10.1128/AEM.69.9.5170 PubMedCentralCrossRefPubMedGoogle Scholar
- Cury JC, Jurelevicius DA, Villela HDM, Jesus HE, Peixoto RS, Schaefer CEGR, Bícego MC, Seldin L, Rosado AS (2015) Microbial diversity and hydrocarbon depletion in low and high diesel-polluted soil samples from Keller Peninsula, South Shetland Islands. Antarct Sci 27:263–273. doi: 10.1017/S0954102014000728 CrossRefGoogle Scholar
- Lane DJ (1991) Nucleic acid techniques in bacterial systematics. In: Stackebrandt E, Goodfellow M (eds) Journal of basic microbiology. Wiley, Chichester, pp 115–175Google Scholar
- Louati H, Said O, Ben SA, Got P, Cravo-Laureau C, Duran R, Aissa P, Pringault O, Mahmoudi E (2014) Biostimulation as an attractive technique to reduce phenanthrene toxicity for meiofauna and bacteria in lagoon sediment. Environ Sci Pollut Res Int 21:3670–3679. doi: 10.1007/s11356-013-2330-5 CrossRefPubMedGoogle Scholar
- Ruberto L, Vazquez S, Mac WP (2008) Bacteriology of extremely cold soils exposed to hydrocarbon pollution. In: Dion P, Nautiyal CS (eds) Microbiology of extreme soils, 13rd edn. Springer, Berlin, pp 247–274Google Scholar
- Tarnocai C, Campbell IB (2002) Soils of the polar regions. In: Dekker M (ed) Encyclopedia of soil science, 2nd edn. Lal R, New York, pp 1018–1021Google Scholar
- Versalovic J, Schneider M, De Bruijn F, Lupski J (1994) Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5:25–40Google Scholar
- Yu Z, García-González R, Schanbacher FL, Morrison M (2008) Evaluations of different hypervariable regions of archaeal 16S rRNA genes in profiling of methanogens by Archaea-specific PCR and denaturing gradient gel electrophoresis. Appl Environ Microbiol 74:889–893. doi: 10.1128/AEM.00684-07 PubMedCentralCrossRefPubMedGoogle Scholar