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Proteomic analysis of conidia germination in Fusarium oxysporum f. sp. cubense tropical race 4 reveals new targets in ergosterol biosynthesis pathway for controlling Fusarium wilt of banana

  • Genomics, transcriptomics, proteomics
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Abstract

Conidial germination is a crucial step of the soilborne fungus Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), a most important lethal disease of banana. In this study, a total of 3659 proteins were identified by isobaric tags for relative and absolute quantitation (iTRAQ)-based comparative proteomic approach, of which 1009 were differentially expressed during conidial germination of the fungus at 0, 3, 7, and 11 h. Functional classification and bioinformatics analysis revealed that the majority of the differentially expressed proteins are involved in six metabolic pathways. Particularly, all differential proteins involved in the ergosterol biosynthesis pathway were significantly upregulated, indicating the importance of the ergosterol biosynthesis pathway to the conidial germination of Foc TR4. Quantitative RT-PCR, western blotting, and in vitro growth inhibition assay by several categories of fungicides on the Foc TR4 were used to validate the proteomics results. Four enzymes, C-24 sterol methyltransferase (ERG6), cytochrome P450 lanosterol C-14α-demethylase (EGR11), hydroxymethylglutaryl-CoA synthase (ERG13), and C-4 sterol methyl oxidase (ERG25), in the ergosterol biosynthesis pathway were identified and verified, and they hold great promise as new targets for effective inhibition of Foc TR4 early growth in controlling Fusarium wilt of banana. To the best of our knowledge, this report represents the first comprehensive study on proteomics profiling of conidia germination in Foc TR4. It provides new insights into a better understanding of the developmental processes of Foc TR4 spores. More importantly, by host plant-induced gene silencing (HIGS) technology, the new targets reported in this work allow us to develop novel transgenic banana leading to high protection from Fusarium wilt and to explore more effective antifungal drugs against either individual or multiple target proteins of Foc TR4.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China No. U1131004, the National High-Tech R&D Program (863 Program) No. 2011AA10020602, 948 Project from the Ministry of Agriculture of China No. 2011-G16, Science and Technology Plan Project of Guangdong Province No. 2011A020201006, and the Pearl River S&T Nova Program of Guangzhou No. 2013J2200081. The authors would like to thank Professor Lai-Liang Cheng and Dr Ted Thannhauser for their critical reading of this manuscript and helpful discussion.

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The authors declare that they have no conflict of interests.

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    Authors and Affiliations

    1. Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China

      Gui-Ming Deng, Qiao-Song Yang, Wei-Di He, Chun-Yu Li, Jing Yang, Cun-Wu Zuo, Jie Gao, Ou Sheng & Gan-Jun Yi

    2. Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China

      Gui-Ming Deng, Qiao-Song Yang, Wei-Di He, Chun-Yu Li, Jing Yang, Cun-Wu Zuo, Jie Gao, Ou Sheng & Gan-Jun Yi

    3. College of Life Sciences, South China Agricultural University, Guangzhou, 510640, China

      Gui-Ming Deng, Jing Yang, Cun-Wu Zuo, Jie Gao, Shao-Yun Lu & Gan-Jun Yi

    4. Key Laboratory of Horticultural Plant Biology of the Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China

      Wei-Di He

    5. Institute of Biotechnology, Cornell University, Ithaca, NY, 14853-2703, USA

      Sheng Zhang

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    Deng, GM., Yang, QS., He, WD. et al. Proteomic analysis of conidia germination in Fusarium oxysporum f. sp. cubense tropical race 4 reveals new targets in ergosterol biosynthesis pathway for controlling Fusarium wilt of banana. Appl Microbiol Biotechnol 99, 7189–7207 (2015). https://doi.org/10.1007/s00253-015-6768-x

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