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Pichia pastoris X-33 has probiotic properties with remarkable antibacterial activity against Salmonella Typhimurium

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Abstract

Probiotics are live microorganisms which are beneficial for the host when ingested at high enough concentrations. The methylotrophic yeast Pichia pastoris is widely used as heterologous protein production platform. However, its use as probiotic is poorly studied. The objective of this study was to evaluate some probiotic properties of the P. pastoris strain X-33 wild type. The resistance to in vitro and in vivo gastrointestinal conditions, stability in feed, safety, and antibacterial activity against Salmonella Typhimurium were evaluated. The yeast remained viable and persisted at appropriate concentration in the diet for at least 2 months, survived the stresses of the gastrointestinal tract in vitro and in vivo, caused no behavioral changes or lesions when administered to mice, inhibited the growth of S. Typhimurium in culture media, and reduced adhesion of the bacteria to the intestinal cells HCT-116. In the challenge experiment with a LD50 of virulent S. Typhimurium strain, mice supplemented with the yeast had a higher survival rate (50 % when administered by gavage and 80 % via the diet, compared with 20 and 50 %, respectively, in the control group). In addition, the S. Typhimurium concentration in the intestine of the surviving mice was lower; the score of intestinal lesions, lower; and the pathogen, not detected in the liver, spleen, and feces when compared to the control group (p < 0.05). It was concluded that the yeast Pichia pastoris X-33 has probiotic properties with remarkable antibacterial activity against S. Typhimurium.

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Acknowledgments

The authors thank professors Odir A. Dellagostin and Vinicius F. Campos for their contribution. They are also indebted to the Brazilian Council for Scientific and Technological Development (CNPq) and Brazilian Federal Agency for Support and Evaluation of Graduate Education (Capes) for funding the project.

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Nucleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Campus Universitário, Capão do Leão, Caixa Postal 354, Pelotas, RS, 96010-900, Brazil

    Rodrigo Correa França, Fabricio Rochedo Conceição, Marcelo Mendonça, Louise Haubert, Gabriela Sabadin, Patrícia Diaz de Oliveira, Marta G. Amaral & Ângela Nunes Moreira

  2. Departamento de Ciência e Tecnologia de Agroindustrial, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas, RS, Brazil

    Rodrigo Correa França, Louise Haubert & Wladimir Padilha da Silva

  3. Departamento de Nutrição, Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil

    Ângela Nunes Moreira

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  1. Rodrigo Correa França
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  2. Fabricio Rochedo Conceição
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  3. Marcelo Mendonça
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  7. Marta G. Amaral
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  9. Ângela Nunes Moreira
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Correspondence to Fabricio Rochedo Conceição.

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França, R.C., Conceição, F.R., Mendonça, M. et al. Pichia pastoris X-33 has probiotic properties with remarkable antibacterial activity against Salmonella Typhimurium. Appl Microbiol Biotechnol 99, 7953–7961 (2015). https://doi.org/10.1007/s00253-015-6696-9

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  • DOI: https://doi.org/10.1007/s00253-015-6696-9

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