Abstract
Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5–C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5–C8) to their respective cyclic alcohols.
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Acknowledgments
We would like to thank Dr. Josef Altenbuchner (Institute of Industrial Genetics—University of Stuttgart) for kindly providing the strain E. coli S17.1 as well as the pCro2a transposon mutagenesis vector.
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Salamanca, D., Karande, R., Schmid, A. et al. Novel cyclohexane monooxygenase from Acidovorax sp. CHX100. Appl Microbiol Biotechnol 99, 6889–6897 (2015). https://doi.org/10.1007/s00253-015-6599-9
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DOI: https://doi.org/10.1007/s00253-015-6599-9