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Comparative genome analysis reveals genetic adaptation to versatile environmental conditions and importance of biofilm lifestyle in Comamonas testosteroni

  • Genomics, transcriptomics, proteomics
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Abstract

Comamonas testosteroni is an important environmental bacterium capable of degrading a variety of toxic aromatic pollutants and has been demonstrated to be a promising biocatalyst for environmental decontamination. This organism is often found to be among the primary surface colonizers in various natural and engineered ecosystems, suggesting an extraordinary capability of this organism in environmental adaptation and biofilm formation. The goal of this study was to gain genetic insights into the adaption of C. testosteroni to versatile environments and the importance of a biofilm lifestyle. Specifically, a draft genome of C. testosteroni I2 was obtained. The draft genome is 5,778,710 bp in length and comprises 110 contigs. The average G+C content was 61.88 %. A total of 5365 genes with 5263 protein-coding genes were predicted, whereas 4324 (80.60 % of total genes) protein-encoding genes were associated with predicted functions. The catabolic genes responsible for biodegradation of steroid and other aromatic compounds on draft genome were identified. Plasmid pI2 was found to encode a complete pathway for aniline degradation and a partial catabolic pathway for chloroaniline. This organism was found to be equipped with a sophisticated signaling system which helps it find ideal niches and switch between planktonic and biofilm lifestyles. A large number of putative multi-drug-resistant genes coding for abundant outer membrane transporters, chaperones, and heat shock proteins for the protection of cellular function were identified in the genome of strain I2. In addition, the genome of strain I2 was predicted to encode several proteins involved in producing, secreting, and uptaking siderophores under iron-limiting conditions. The genome of strain I2 contains a number of genes responsible for the synthesis and secretion of exopolysaccharides, an extracellular component essential for biofilm formation. Overall, our results reveal the genomic features underlying the adaption of C. testosteroni to versatile environments and highlighting the importance of its biofilm lifestyle.

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Acknowledgments

The authors thank Daphne Ng for her helpful suggestions. This research was supported by the National Research Foundation and Ministry of Education Singapore under its Research Centre of Excellence Programme, Singapore Centre on Environmental Life Sciences Engineering (SCELSE) (M4330005.C70), and a Start-up Grant (M4080847.030) from College of Engineering, Nanyang Technological University, Singapore. The authors thank the Singapore Ministry of Education (MOE2011-T2-2-035, ACR 3/12) for the research scholarship to Yichao Wu.

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  1. School of Civil and Environmental Engineering, Nanyang Technological University, Singapore, 639798, Singapore

    Yichao Wu, Anee Mohanty & Bin Cao

  2. Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore

    Yichao Wu, Krithika Arumugam, Martin Qi Xiang Tay, Hari Seshan, Anee Mohanty & Bin Cao

  3. Department of Civil and Environmental engineering, University of California, Davis, Davis, CA, USA

    Hari Seshan

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Wu, Y., Arumugam, K., Tay, M.Q.X. et al. Comparative genome analysis reveals genetic adaptation to versatile environmental conditions and importance of biofilm lifestyle in Comamonas testosteroni . Appl Microbiol Biotechnol 99, 3519–3532 (2015). https://doi.org/10.1007/s00253-015-6519-z

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