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Chilli leaf curl virus infection highlights the differential expression of genes involved in protein homeostasis and defense in resistant chilli plants

Applied Microbiology and Biotechnology volume 99, pages 4757–4770 (2015)Cite this article

Abstract

Geminiviruses have evolved with tremendous potential of recombination and possess the ability to manipulate several cellular processes of hosts. Chilli leaf curl virus (ChiLCV) is a monopartite Begomovirus (family Geminiviridae) which has emerged as a serious threat to chilli production worldwide. To date, development of resistant chilli varieties through conventional plant breeding techniques remains the major antiviral strategy. To explore the potential resistance factors in Capsicum annuum var. Punjab Lal, we performed a transcriptome analysis in ChiLCV-infected plants by exploiting the advantage of sensitivity and efficiency of suppression subtractive hybridization (SSH). Out of 480 clones screened, 231 unique expressed sequence tags (ESTs) involved in different cellular and physiological processes were identified. An interactome network of ChiLCV responsive differentially expressed genes revealed an array of proteins involved in key cellular processes including transcription, replication, photosynthesis, and defense. A comparative study of gene expression between resistant and susceptible chilli plants revealed upregulation of several defense-related genes such as nucleotide-binding site leucine-rich repeat (NBS-LRR) domain containing protein, lipid transfer protein, thionin, polyphenol oxidase, and other proteins like ATP/ADP transporter in the ChiLCV-resistant variety. Taken together, the present study provides novel insights into the transcriptomics of ChiLCV-resistant chilli plants.

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Acknowledgments

Nirbhay Kushwaha thanks the Council of Scientific and Industrial Research for providing a senior research fellowship. The authors gratefully acknowledge the financial support from the Department of Biotechnology, India (grant no. BT/PR/6504/AGII/106/878/2012). We thank Dr. V. K. Sharma and Ms. Mansi Bhardwaj for their suggestions and critical comments on the manuscript.

Conflict of interest

The authors declare that they do not have any conflict of interest.

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Affiliations

  1. Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India

    Nirbhay Kushwaha, Pranav Pankaj Sahu & Supriya Chakraborty

  2. National Institute of Plant Genome Research, New Delhi, 110067, India

    Manoj Prasad

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  1. Nirbhay Kushwaha
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  2. Pranav Pankaj Sahu
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  3. Manoj Prasad
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  4. Supriya Chakraborty
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Correspondence to Supriya Chakraborty.

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Kushwaha, N., Sahu, P.P., Prasad, M. et al. Chilli leaf curl virus infection highlights the differential expression of genes involved in protein homeostasis and defense in resistant chilli plants. Appl Microbiol Biotechnol 99, 4757–4770 (2015). https://doi.org/10.1007/s00253-015-6415-6

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  • DOI: https://doi.org/10.1007/s00253-015-6415-6

Keywords

  • Geminivirus
  • Transcriptome analysis
  • Host gene expression
  • Resistance
  • Chilli
  • Suppression subtractive hybridization